高迁移率族蛋白1在过敏性紫癜患儿血清中的表达及意义

Expression and clinical significance of serum high-mobility group protein box 1 in children with Henoch-Schönlein purpura

摘要:

目的 检测过敏性紫癜(HSP)患儿外周血高迁移率族蛋白1(HMGB1 )、超敏C反应蛋白(hs-CRP)及D-二聚体(D-D )的表达,探讨HMGB1在HSP患儿中的意义。方法 HSP儿童(HSP组)40例和健康儿童(对照组)30例作为研究对象。采用酶联免疫吸附法检测血清HMGB1的水平,全自动生化分析仪和全自动血凝分析仪检测血清hs-CRP及血浆D-D 水平。结果 HSP组外周血急性期HMGB1、hs-CRP 、D-D 含量均较对照组增高,差异有统计学意义(均P< 0.05);伴有肾损害者其含量高于无肾损害者(P< 0.05)。HSP患儿HMGB1表达水平与hs-CRP及D-D 的表达均呈正相关(分别r=0.878、0.625,均P< 0.001)。结论 HMGB1的表达与HSP患儿的炎症反应及高凝状态有关,HMGB1可能参与了儿童HSP及HSP肾损害的发病。

Abstract:

Objective To determine the expression of high-mobility group protein box 1 (HMGB1), high-sensitivity C-reactive protein (hs-CRP), and D-dimer (D-D) in the peripheral blood of children with Henoch-Schönlein purpura (HSP) and to investigate the clinical significance of HMGB1 in children with HSP. Methods A total of 40 children with HSP (HSP group) and 30 healthy children (control group) were involved in the study. The level of serum HMGB1 was determined using enzyme-linked immunosorbent assay, and the levels of serum hs-CRP and plasma D-D were determined using automatic biochemical analyzer and automatic blood coagulation analyzer, respectively. Results The levels of HMGB1, hs-CRP, and D-D in the peripheral blood of the HSP group in the acute phase were significantly higher than in the control group (P< 0.05). The levels of the three indicators were significantly higher in HSP children with renal damage than in those without renal damage (P< 0.05). In children with HSP, the expression of HMGB1 was positively correlated with the expression of hs-CRP and D-D (r=0.878, P< 0.001; r=0.625, P< 0.001). Conclusions The expression of HMGB1 is related to the inflammatory response and hypercoagulability in children with HSP. HMGB1 may be involved in the development of HSP and associated renal damage in children.