2',5'-寡腺苷酸合成酶1基因多态性与自发性早产和胎膜早破易感性的病例对照研究

A case-control study on association between OAS1 polymorphism and susceptibility to spontaneous preterm birth and preterm premature rupture of membranes

摘要:

目的 探讨2',5'-寡腺苷酸合成酶1(OAS1)基因多态性和自发性早产和胎膜早破易感性的关系。方法 收集自发性早产儿599 例作为病例组,其中包括胎膜早破171 例;孕母无自发性早产和胎膜早破病史的673 例足月儿作为对照组。采用病例-对照研究,用聚合酶链反应-限制性片段长度多态性分析检测OAS1 内含子区rs10774671 位点的单核苷酸多态性。结果 病例组和对照组两组间OAS1 rs10774671 基因型AA、GA、GG 以及等位基因A、G 的频率之间的差异均无统计学意义;有无胎膜早破病例组分别和对照组比较,上述频率之间的差异均无统计学意义;不同胎龄的自发性早产亚组分别和对照组比较,上述频率之间的差异均无统计学意义。结论 OAS1 基因多态性与自发性早产和胎膜早破易感性之间缺乏关联。

Abstract:

Objective To investigate the association between the genetic polymorphism of 2',5'-oligoadenylate synthetase 1 (OAS1) and susceptibility to spontaneous preterm birth (SPTB) and preterm premature rupture of membranes (PPROM).Methods The case-control study consisted of 599 preterm infants including 171 cases of PPROM, and 673 full-term infants without maternal histories of SPTB and PPROM as controls. The single nucleotide polymorphism (SNP) at OAS1 intron 5, rs10774671, was analyzed by polymerase chain reaction-restriction fragment length polymorphism.Results No significant differences were observed between the case and control groups in the frequencies of genotypes (AA, GA, and GG) and alleles (A and G) of OAS1 rs10774671. When the case group was divided into two subgroups with or without PPROM, no significant differences in the genotype and allele frequencies were found between each subgroup and the control group. When the case group was divided into three subgroups with different gestational ages at SPTB, no significant differences in the genotype and allele frequencies were detected between each subgroup and the control group.Conclusions No association is identified between OAS1 SNP and susceptibility to SPTB and PPROM.