肺炎支原体抗体和载量指数在儿童肺炎支原体肺炎诊断中的应用

Application of Mycoplasma pneumoniae antibody and load index in the diagnosis of Mycoplasma pneumoniae pneumonia in children

摘要:

目的 评估肺炎支原体载量指数（MPLI）与血清总抗体在MP 肺炎诊断中的价值。方法 选取115 例肺炎支原体肺炎患儿和400 例健康儿童为研究对象，荧光定量PCR 与被动凝集法检测MP 肺炎患儿不同病程阶段的MP-DNA 及总抗体水平，并计算MPLI。结果 肺炎支原体肺炎患儿的MPLI 临界值为6.12。MPLI和MP 总抗体滴度均随病程升高，MP-DNA 则随病程迅速下降；MP-DNA 载量较高组的总抗体滴度显著高于MP载量较低组（P < 0.05）。病程2 周内抗体阴性组的MPLI 显著大于抗体阳性组（P < 0.05）。结论 MPLI 提供了标准化的MP-DNA 定量值，对肺炎支原体感染的早期诊断有重要价值。

Abstract:

Objective To study the role of Mycoplasma pneumoniae (MP) load and antibody measurements in the diagnosis of MP pneumonia. Methods A total of 115 children with MP pneumonia and 400 healthy children were enrolled. The MP load and total antibody level were measured at different stages, and the MP load index (MPLI) was calculated. Results The cut-off value of MPLI for MP infection was 6.12. MPLI and total antibody titer increased during the course of the disease, while MP-DNA decreased rapidly. Within the same time of blood collection, the group with a higher MP load had a significantly higher total antibody titer than the group with a lower MP load (P < 0.05). Within 2 weeks of the course of the disease, the negative antibody group had a significantly higher MPLI than the positive antibody group (P < 0.05). Conclusions MPLI provides a standardized quantitative value of MP-DNA and plays an important role in the early diagnosis of MP infection.