IL-19基因多态性与儿童乙型肝炎病毒易感性研究

Association between IL-19 gene polymorphisms and hepatitis B virus susceptibility in children

摘要:

目的 探讨白介素19（IL-19）基因的单核苷酸多态性（SNP）与儿童乙型肝炎病毒（HBV）易感性的关系。方法 采用病例对照研究，收集HBsAg阳性儿童136例（病例组），HBsAg阴性的健康儿童297例（对照组），应用PCR 聚合酶链反应和DNA测序法进行基因分型。结果 IL-19基因的rs1798位点的基因型在病例组和对照组人群中频率分布差异有统计学意义，病例组的CG 基因型的比例显著高于对照组（P < 0.05）；IL-19基因的rs2243191位点的基因型和等位基因在HBV感染高危儿童感染组和未感染组人群中频率分布差异有统计学意义，感染组TC、CC基因型以及C等位基因的比例显著高于未感染组（P < 0.05）。结论 IL-19 基因SNP 位点rs1798的基因多态性可能与儿童乙型肝炎易感性相关；rs2243191的基因多态性可能与HBV感染高危儿童突破感染易感性相关。

Abstract:

Objective To study the association between the single nucleotide polymorphisms (SNP) of interleukin (IL)-19 and susceptibility to hepatitis B virus (HBV) infection in children. Methods A case-control study was performed, and 136 children with positive HBsAg (case group) and 297 healthy children with negative HBsAg (control group) were enrolled. PCR and DNA sequencing were used for genotyping. Results There were significant differences in the frequencies of genotypes of IL-19 rs1798 between the case and control groups. The case group also had a significantly higher proportion of children with CG genotype than the control group (P < 0.05). There were significant differences in the frequencies of genotypes and alleles of IL-19 rs2243191 between the HBV infection and non-infection groups among children who born to HBV-positive mothers. The infection group had significantly higher proportions of children with TC and CC genotypes and C allele than the non-infection group (P < 0.05). Conclusions The SNP of IL-19 rs1798 may be associated with susceptibility to hepatitis B in children, and the SNP of IL-19 rs2243191 may be associated with susceptibility to breakthrough HBV infection in children at a high risk of HBV infection.