microRNA-296-5p靶基因的预测及在肺发育中的应用

Prediction of microRNA-296-5p target genes and its application in lung development

摘要:

目的 对rno-microRNA-296-5p（miR-296）靶基因预测并进行相关生物信息学分析，为深入研究miR-296与胎肺发育相关的生物学功能提供理论依据。方法 应用PubMed、Google等信息搜索工具检索miR-296的现有研究，并通过miRBase获取其序列特征以及进化的保守性；利用TargetScan数据库进行miR-296的靶基因预测；并利用DAVID Bioinformatics Resources 6.8数据库对靶基因进行功能富集分析；利用KEGG数据库对靶基因进行信号转导通路分析。结果 已有的文献表明miR-296在许多生物学过程中发挥重要作用，序列在多物种间高度保守。miR-296调控的靶基因参与分子功能、细胞组分和生物学过程等方面，靶基因集合的信号转导通路显著富集于MAPK信号通路、Wnt信号通路、TGF-β信号通路等（均P < 0.05）。结论 通过对miR-296靶基因的生物信息学分析，为研究miR-296在肺发育中的作用提供了功能与机制的线索。

Abstract:

Objective To predict the target genes of rno-microRNA-296-5p (miR-296) using bioinformatics software and databases, and to provide a theoretical basis for further studies of biological effects of miR-296 in fetal lung development. Methods PubMed and Google were used to search for all reported literature on miR-296. The miRBase database was used to determine the sequence and evolutionary conservatism of miR-296. The TargetScans database was used to predict the target genes of miR-296. The DAVID Bioinformatics Resources 6.8 database was used for the functional enrichment analysis of the target genes. The KEGG database was used to analyze the signaling pathways of target genes. Results miR-296 was reported to play important roles in many biological processes and have a high degree of sequence conservation among species. The target genes of miR-296 were involved in biological processes, cell components, and molecular function. Those target genes were significantly enriched in the mitogen-activated protein kinase signaling pathway, Wnt signaling pathway, and transforming growth factor-β signaling pathway (P < 0.05). Conclusions The bioinformatics analysis of the target genes of miR-296 provides a basis for studying biological effects and mechanism of action of miR-296 in lung development.