IL1R1基因多态性与儿童哮喘的相关性

Association between IL1R1 gene polymorphisms and childhood asthma

摘要:

目的 探讨我国中部地区儿童IL1R1 基因的两个SNP 位点(rs1558641 和rs949963)的多态性与哮喘易感性的相关性。方法 采用病例-对照的研究方法,选取来自于我国中部地区的208 例哮喘患儿(哮喘组)和223 例同时期体检正常的儿童(健康对照组)作为研究对象。利用限制性片段长度多态性分析(PCRRFLP)的方法检测IL1R1 基因两个SNP 位点(rs1558641 和rs949963)的多态性分布;酶联免疫吸附试验(ELISA)测定血清中IL1R1 的水平。结果 哮喘组患儿SNP 位点(rs1558641)的基因型及等位基因频率与健康对照组相比差异无统计学意义。而哮喘组患儿SNP 位点(rs949963)GG 基因型的比例显著高于健康对照组(P=0.031),且两组等位基因频率差异也有统计学意义(P=0.018)。哮喘组血清IL1R1 的水平明显高于健康对照组(P=0.011),且SNP 位点(rs949963)GG 基因型的患儿血清IL1R1 水平高于其他基因型(AA+AG)的患儿(P=0.028)。结论 IL1R1 基因SNP 位点(rs949963)的多态性与我国中部地区儿童哮喘的易感性相关,且该位点的多态性可能影响患儿血清中IL1R1 的表达水平。

Abstract:

Objective To investigate the association of two single-nucleotide polymorphisms (SNPs) in IL1R1 gene (rs1558641 and rs949963) with the susceptibility to asthma in children from Central China. Methods A casecontrol study was performed in the asthma group and the control group, consisting of 208 children with asthma and 223 normal children from Central China, respectively. The genotypes of two SNPs in IL1R1 gene, rs1558641 and rs949963, were identified using polymerase chain reaction-restriction fragment length polymorphism. The serum level of IL1R1 was determined by enzyme-linked immunosorbent assay. Results There were no significant differences in genotype and allele frequencies of rs1558641 between the asthma and control groups. In terms of rs949963, the frequencies of GG genotype and alleles were significantly higher in the asthma group than in the control group (P<0.05). The asthma group had a significantly higher serum level of IL1R1 than the control group (P=0.011). Moreover, the serum level of IL1R1 was significantly higher in patients with GG genotype than in those with AA or AG genotype for rs949963 (P=0.028). Conclusions IL1R1 SNP rs949963 is associated with the susceptibility to asthma in children from Central China and may increase the serum expression of IL1R1.