葡萄糖-6-磷酸脱氢酶缺乏症基因启动子甲基化研究

Gene promoter methylation in glucose-6-phosphate dehydrogenase de?ciency

摘要:

目的 了解葡萄糖-6-磷酸脱氢酶(G6PD)基因启动子区甲基化改变特点,探讨甲基化改变与G6PD缺乏症的关系。方法 将2013年8月至2014年7月期间195例贫血查因或体检儿童分为G6PD缺乏组(130例)和对照组(65例)。荧光定量PCR检测G6PDmRNA表达情况。甲基化敏感性高分辨率熔解曲线分析(MS-HRM)结合重亚硫酸盐PCR测序法(BSP)等方法分析G6PDmRNA表达偏低的G6PD缺乏症患者的基因启动子甲基化改变情况,另对G6PDmRNA表达正常的44例女性标本(G6PD缺乏组7例和正常对照组37例)的G6PD基因启动子甲基化情况进行分析。结果 22例(16.9%,22/130)G6PD缺乏症患者的G6PDmRNA表达偏低,其中的16例男性均无甲基化、6例女性均存在部分甲基化。44例G6PDmRNA表达正常的G6PD缺乏组及对照组女性中,40例检测到部分甲基化,4例无甲基化(G6PD缺乏组1例,对照组3例)。结论 基因启动子区甲基化与男性G6PD缺乏症没有关联。女性人群G6PD基因存在部分甲基化与无甲基化,提示甲基化改变对女性G6PD缺乏症可能有一定影响。

Abstract:

Objective To investigate the features of methylation in the promoter region of glucose-6-phosphate dehydrogenase (G6PD) gene and the association between gene promoter methylation and G6PD deficiency. Methods Fluorescent quantitative PCR was used to measure the mRNA expression of G6PD in 130 children with G6PD deficiency. Sixty-five children without G6PD deficiency served as the control group. The methylation-sensitive high-resolution melting curve analysis and bisulfite PCR sequencing were used to analyze gene promoter methylation in 22 children with G6PD deficiency and low G6PD mRNA expression. The G6PD gene promoter methylation was analyzed in 44 girls with normal G6PD mRNA expression (7 from G6PD deficiency group and 37 from control group). Results Twenty-two (16.9%) children with G6PD deficiency had relatively low mRNA expression of G6PD; among whom, 16 boys showed no methylation, and 6 girls showed partial methylation. Among the 44 girls with normal G6PD mRNA expression, 40 showed partial methylation, and 4 showed no methylation (1 case in the G6PD group and 3 cases in the control group). Conclusions Gene promoter methylation is not associated with G6PD deficiency in boys. Girls have partial methylation or no methylation in the G6PD gene, suggesting that the methylation may be related to G6PD deficiency in girls.