新生猪溶血性黄疸模型的制备与验证

Establishment and validation of a neonatal pig model of hemolytic jaundice

摘要:

目的 建立新生猪溶血性黄疸动物模型,以进一步研究新生儿溶血性黄疸的病理生理。方法 7日龄纯种大约克白猪分成实验组和对照组,每组各6只。并采用免疫新西兰大白兔的方法 制备兔抗猪红细胞抗体,分离兔抗猪红细胞血清。实验组静脉注射兔抗猪红细胞血清5mL,对照组注射5mL生理盐水。两组均每6h采血送检血常规及肝功能。结果 实验组注射兔抗猪红细胞血清18h后血清胆红素水平高于对照组(64±30μmol/Lvs20±4μmol/L,P<0.05),48h达高峰(275±31μmol/L),96h明显降低(95±17μmol/L),均高于对照组(P<0.05)。注射抗血清后18h,实验组红细胞降至(4.58±0.32)×10¹²/L,低于对照组[(5.09±0.44)×10¹²/L](P<0.05);24h实验组红细胞[(4.21±0.24)×10¹²/L]和HB(87±3g)进一步降低,低于对照组[(5.11±0.39)×10¹²/L,97±6g](P<0.05);二组间的红细胞、血红蛋白差异在36~48h达高峰。结论 新生猪溶血性黄疸模型较好地模拟了人类溶血性黄疸的病理过程,为更进一步研究新生儿溶血病提供了良好的生物物质基础。

Abstract:

Objective To establish a neonatal pig model of hemolytic jaundice. Methods Twelve sevenday-old purebred Yorkshire pigs were randomly divided into an experimental group and a control group (n=6 each). Immunization of New Zealand white rabbits was used to prepare rabbit anti-porcine red blood cell antibodies, and rabbit anti-porcine red blood cell serum was separated. The neonatal pigs in the experimental group were given an intravenous injection of rabbit anti-porcine red blood cell serum (5 mL), and those in the control group were given an intravenous injection of normal saline (5 mL). Venous blood samples were collected every 6 hours for routine blood test and liver function evaluation. Results The experimental group had a significantly higher serum bilirubin level than the control group at 18 hours after the injection of rabbit anti-porcine red blood cell serum (64±30 μmol/L vs 20±4 μmol/L; P<0.05). In the experimental group, the serum bilirubin level reached the peak at 48 hours (275±31 μmol/L), and decreased significantly at 96 hours after the injection (95±17 μmol/L), but all significantly higher than that in the control group (P<0.05). At 18 hours after the injection, the experimental group had a significantly lower red blood cell (RBC) count than the control group [(4.58±0.32)×10¹²/L vs (5.09±0.44)×10¹²/L; P<0.05]; at 24 hours, the experimental group showed further reductions in RBC count and hemoglobin level and had significantly lower RBC count and hemoglobin level than the control group [RBC: (4.21±0.24)×10¹²/L vs (5.11±0.39)×10¹²/L, P<0.05; hemoglobin: 87±3 g vs 97±6 g, P<0.05]. The differences in RBC count and hemoglobin level between the two groups were largest at 36-48 hours. Conclusions The neonatal pig model of hemolytic jaundice simulates the pathological process of human hemolytic jaundice well and provides good biological and material bases for further investigation of neonatal hemolysis.