多巴胺D2受体基因rs4274224多态性及家庭因素对学龄儿童节律性的影响

Effects of rs4274224 polymorphisms in the DRD2 gene and family factors on the regularity based on the temperament in school-age children

摘要:

目的 探讨多巴胺D2受体(DRD2)基因rs4274224多态性、家庭因素与学龄儿童节律性的关联及交互作用。方法 采用8~12岁儿童气质问卷(MCTQ)、父母教养方式评价量表(EMBU)、家庭亲密度与适应性量表(FACESⅡ-CV)对儿童节律性、父母教养方式及家庭功能进行测评,运用自动化Mass Array SNP基因型分型技术分析DRD2基因rs4274224多态性。结果 DRD2基因rs4274224位点 AA基因型儿童的节律性得分(2.9±0.6分)明显低于GA/GG基因型儿童(3.1±0.7分),差异有统计学意义 (P < 0.05)。多元回归分析显示,儿童性别、父亲文化程度、家庭适应性对儿童节律性得分有影响 (P < 0.05)。Logistic 回归分析显示,影响儿童节律性高低的主要因素为家庭适应性、DRD2基因rs4274224多态性与家庭适应性的交互作用,高适应性家庭的儿童节律性更高(OR = 0.112,P < 0.01),家庭适应性低增加AA基因型儿童发生节律性问题的风险(OR = 21.554,P < 0.01)。结论 家庭适应性及其与rs4274224的交互作用共同作用于儿童节律性。

Abstract:

Objective To study the effects of rs4274224 polymorphisms in the DRD2 gene, family factors and their interaction on the regularity in school-age children. Methods The rs4274224 polymorphisms were genotyped using Sequenom Mass Array. The regularity was assessed based on the Middle Childhood Temperament Questionnaire (MCTQ). The parental rearing pattern was assessed with Egna Minnen av Bardnodnauppforstran (EMBU). The family function was assessed using Family Cohesion and Adaptability Scale (FACES II-CV). Results The regularity score in children with AA genotype of rs4274224 in the DRD2 gene was signifificantly lower than in those with GA/GG genotype (2.9±0.6 vs 3.1±0.7; P < 0.05). The results of multiple regression analysis showed that the regularity was related to child gender, father's education level and family adaptability. The results of logistic regression analysis showed that the main factors influencing the regularity were family adaptability and its interaction with rs4274224 polymorphisms. The regularity was better in children with high family adaptability than in those with low family adaptability (OR = 0.112, P < 0.01). The children with AA genotype and low family adaptability were tend to be associated with low regularity (OR = 21.554, P < 0.01). Conclusions The regularity based the temperament for school-age children might be influenced by family adaptability and its interaction with rs4274224 polymorphisms.