PI3K、NF-κB 和STAT1在支气管哮喘儿童外周血单个核细胞的表达和临床意义

Expression profiles of PI3K, NF-κB, and STAT1 in peripheral blood mononuclear cells in children with bronchial asthma

摘要:

目的 检测PI3K、NF-κB和STAT1在支气管哮喘儿童外周血单个核细胞 （PBMC）中的表达，探讨其在哮喘发病机制的临床意义。方法 选取30例急性发作期的支气管哮喘患儿作为哮喘组，随机选取同期健康儿童20例作为对照组。应用RT-PCR和Westernblot方法检测PBMC中PI3K、NF-κB和STAT1的mRNA和蛋白表达，肺功能仪检测两组肺功能，并分析支气管哮喘儿童PI3K、NF-κB和STAT1的mRNA水平与其肺功能的相关性。结果 哮喘组的PI3K、NF-κB和STAT1的mRNA和蛋白水平均高于对照组 （P <0.05）。与对照组相比，哮喘组FEV1%、FEV1/FVC、PEF%等肺功能指标均下降 （P <0.05）。哮喘儿童的PI3K、NF-κB和STAT1的mRNA表达水平与FEV1%、FEV1/FVC、PEF%均呈负相关 （P <0.05）。结论 哮喘患儿的PI3K、NF-κB和STAT1表达上调并与肺功能呈负相关，三者可能共同参与儿童支气管哮喘的发生、发展。

Abstract:

Objective To study the expression profiles of PI3K, NF-κB, and STAT1 in peripheral blood mononuclear cells (PBMCs) in children with bronchial asthma, as well as their roles in the pathogenesis of asthma. Methods Thirty children with acute exacerbation of bronchial asthma were enrolled as the asthma group, and 20 healthy children were enrolled as the control group. RT-PCR and Western blot were used to measure the mRNA and protein expression levels of PI3K, NF-κB, and STAT1 in PBMCs. A spirometer was used to compare the pulmonary function between the two groups. The correlations between the mRNA expression of PI3K, NF-κB, and STAT1 and pulmonary function in children with bronchial asthma were analyzed. Results The asthma group had significantly higher mRNA and protein expression levels of PI3K, NF-κB, and STAT1 than the control group (P<0.05). Compared with the control group, the asthma group showed significant reductions in pulmonary function indices such as FEV1%, FEV1/FVC, and PEF% (P<0.05). In children with bronchial asthma, the mRNA expression levels of PI3K, NF-κB, and STAT1 were negatively correlated with FEV1%, FEV1/FVC, and PEF% (P<0.05). Conclusions The expression levels of PI3K, NF-κB, and STAT1 increase in children with asthma, and are negatively correlated with pulmonary function indices, suggesting that PI3K, NF-κB and STAT1 are involved in the development and progression of bronchial asthma in children.