脂肪间充质干细胞移植对食物过敏小鼠免疫干预作用

Immunoregulatory effect of adipose-derived stem cell transplantation in young mouse model of food allergy

摘要:

目的 探讨在致敏前和致敏后脂肪间充质干细胞 （ADSC）腹腔注射移植对Balb/c小鼠食物过敏的免疫干预作用。方法 将32只3周龄Balb/c雌性小鼠随机分为空白对照组、过敏模型组、ADSC治疗组和ADSC预防组，每组8只。利用卵清蛋白 （OVA）腹腔注射致敏建立小鼠食物过敏模型，ADSC预防组和治疗组分别在造模第1天和第15天进行干细胞移植 （1×106个细胞数）。观察各组小鼠相关的过敏症状并进行评分；苏木精-伊红染色观察各组小鼠空肠绒毛及炎性细胞浸润情况；采用Luminex液相芯片技术检测各组小鼠血清中过敏相关的细胞因子水平变化。结果 与过敏模型组相比，ADSC治疗组和预防组过敏症状评分均明显降低 （P<0.05），同时这两组的空肠黏膜过敏性病理损伤显著减轻。与过敏模型组比较，ADSC预防组和治疗组IL-4、IL-5、IL-6、IL-17A、IL-22、IL-23水平显著下降 （P<0.05）。与过敏模型组和ADSC预防组比较，ADSC治疗组IFN-γ水平升高，MCP-1水平下降 （P<0.05）。结论 ADSC移植对致敏前或致敏后的Balb/c幼鼠食物过敏均有免疫干预作用，致敏后ADSC移植比致敏前移植免疫调节效果更好。

Abstract:

Objective To investigate the immunoregulatory effect of adipose-derived stem cell (ADSC) transplantation by intraperitoneal injection in food-allergic young mice before and after ovalbumin (OVA) sensitization. Methods Thirty-two 3-week-old female Balb/c mice were randomly divided into control, allergic model, ADSC treatment, and ADSC prevention groups (n=8 each). A young mouse model of food allergy was established by OVA sensitization via intraperitoneal injection. Each mouse from the prevention and treatment groups was transplanted with 1×106 ADSCs on days 1 and 15 post-sensitization, respectively. The allergic symptoms of all groups were observed and scored. The jejunal villi and inflammatory cell infiltration were observed by hematoxylin-eosin staining. Serum levels of allergy-related inflammatory cytokines were measured by Luminex. Results Compared with the allergic model group, the ADSC prevention and ADSC treatment groups had significantly reduced allergic symptom scores (P<0.05). The two groups also showed significantly alleviated allergic pathological damage of the jejunal mucosa. Serum levels of interleukin (IL)-4, IL-5, IL-6, IL-17A, IL-22 and IL-23 were significantly lower in the ADSC prevention and treatment groups than in the allergic model group (P<0.05). However, the ADSC treatment group had a significantly increased serum interferon-γ level and a significantly reduced serum monocyte chemotactic protein-1 level compared with the allergic model and ADSC prevention groups (P<0.05). Conclusions ADSC transplantation, performed before or after sensitization, has an immunoregulatory effect on food allergy in young Balb/c mice, but this effect is better if transplantation is performed after sensitization.