婴幼儿腹泻相关腺病毒的基因型研究

Genotypes of adenoviruses in infants and young children with diarrhea

摘要:

目的 探讨婴幼儿腹泻患者腺病毒 （AdV） 的分布情况及基因型特点。方法 选取3岁以下腹泻患儿380例，提取粪便基因组DNA进行腺病毒PCR检测，并对检测阳性的进行克隆测序和基因型分析。结果 在380例样本中，共检出24例腺病毒，检出率为6.3% （24/380）。阳性病例中以2~3岁患儿为主。阳性标本病毒序列分析显示，肠道腺病毒41型检出率为4.2% （16/380），非肠道腺病毒检出率为2.1% （8/380）：其中AdV1型2例，AdV2型2例，AdV7型1例，AdV12型2例，AdV31型1例。结论 婴幼儿腺病毒腹泻常见于2~3岁的婴幼儿，AdV41是主要流行优势株。

Abstract:

Objective To investigate the prevalence of adenoviruses (AdV) and their genotypes in infants and young children with diarrhea. Methods A total of 380 children with diarrhea aged less than 3 years were enrolled. The genomic DNA was extracted from stool and PCR was used to detect AdV. Clone sequencing and genotyping were performed for DNA in AdV-positive specimens. Results AdV was detected in 24 out of 380 specimens, and the detection rate was 6.3% (24/380). A majority of children with positive AdV were aged 2-3 years. The viral sequence analysis of positive specimens showed that the detection rates of enteric AdV41 and non-enteric AdV were 4.2% (16/380) and 2.1% (8/380), respectively, and among the children with non-enteric AdV, there were 2 with AdV1, 2 with AdV2, 1 with AdV7, 2 with AdV12, and 1 with AdV31. Conclusions Diarrhea caused by AdV is commonly seen in children aged 2-3 years, and AdV41 is the major predominant strain.