哮喘患儿外周血中CD4+LAP+调节性T细胞比例及功能变化研究

Changes in proportion and function of peripheral CD4+LAP+ regulatory T cells in children with asthma

摘要:

目的 测定哮喘患儿外周血中CD4⁺LAP⁺调节性T细胞 （CD4⁺LAP⁺Treg细胞） 比例和功能的改变，探讨CD4⁺LAP⁺Treg细胞在哮喘发病中的作用。方法 选取2014年3月至2015年9月确诊为哮喘的患儿75例为研究对象，依据病情将哮喘患儿分为哮喘急性期组 （n=40） 和哮喘缓解期组 （n=35）；另选取行健康体检儿童30例为健康对照组。采用流式细胞仪检测各组儿童外周血CD4⁺LAP⁺Treg细胞百分比；采用3H-脱氧胸腺嘧啶苷法检测各组CD4⁺LAP⁺Treg细胞的免疫抑制情况。结果 哮喘急性期组患儿体内CD4⁺LAP⁺Treg比例 （2.0%±1.0%） 较缓解期组 （4.1%±2.4%） 及健康对照组 （4.6%±3.0%） 均降低 （P < 0.05）；哮喘急性期组患儿体内CD4⁺LAP⁺Treg细胞免疫抑制率 （21%±4%） 较缓解期组 （55%±9%） 及健康对照组 （62%±11%） 均降低 （P < 0.05）。结论 哮喘患儿外周血中CD4⁺LAP⁺Treg数量降低和抑制功能减弱可能参与了哮喘的急性发病过程。

Abstract:

Objective To investigate the changes in the proportion and function of peripheral CD4⁺LAP⁺regulatory T cells (CD4⁺LAP⁺Treg cells) in children with asthma, as well as the role of CD4⁺LAP⁺Treg cells in the pathogenesis of asthma. Methods A total of 75 children who were diagnosed with asthma from March 2014 to September 2015 were enrolled as study subjects, and according to their conditions, they were divided into acute-stage asthma group (40 children) and remission-stage asthma group (35 patients). Another 30 children who underwent physical examination were enrolled as the healthy control group. Flow cytometry was used to determine the percentage of peripheral CD4⁺LAP⁺Treg cells, and[3H]-thymidine incorporation assay was performed to analyze the immunosuppression of CD4⁺LAP⁺Treg cells in each group. Results The acute-stage asthma group showed significant reductions in the proportion of CD4⁺LAP⁺Treg cells compared with the remission-stage asthma group and the healthy control group (2.0%±1.0% vs 4.1%±2.4%/4.6%±3.0%; P < 0.05). The acute-stage asthma group also showed a significant reduction in the immunosuppression rate of CD4⁺LAP⁺Treg cells compared with the remission-stage asthma group and the healthy control group (21%±4% vs 55%±9%/62%±11%; P < 0.05). Conclusions In children with asthma, the reduction in the number and inhibitory function of peripheral CD4⁺LAP⁺Treg cells may be involved in the pathogenesis of asthma.