Wnt通路中Wif-1和β-catenin在儿童急性淋巴细胞白血病中的表达

Expression of Wif-1 and β-catenin in the Wnt pathway in childhood acute lymphoblastic leukemia

摘要:

目的 研究Wnt通路中Wnt通路抑制因子1 （Wif-1）和β连环蛋白 （β-catenin）在儿童急性淋巴细胞白血病 （ALL）的表达及可能的作用。方法 收集初发并且诱导缓解治疗第33天完全缓解的35例ALL患儿的临床资料，以治疗前作为初发组，第33天达完全缓解时作为缓解组，对照组为15例非恶性血液病患儿。RT-PCR方法检测Wif-1和β-catenin mRNA的表达，ELISA法测Wif-1蛋白的表达。结果 初发组Wif-1 mRNA及蛋白的表达明显低于对照组和缓解组，β-catenin mRNA的表达高于对照组和缓解组 （P < 0.05）。在初发组和缓解组，高危患儿的β-catenin mRNA表达均高于中危和低危患儿，Wif-1 mRNA、蛋白表达低于中危和低危患儿 （P < 0.05）。在初发组和缓解组，T-ALL患儿β-catenin mRNA的表达高于B-ALL患儿，Wif-1 mRNA、蛋白表达低于B-ALL患儿 （P < 0.05）。各组Wif-1和β-catenin mRNA表达呈负相关 （P < 0.05）。结论 Wif-1表达下降、β-catenin表达增高是儿童急淋发病机制之一，Wif-1下降和/或β-catenin增高的程度可能与预后相关。

Abstract:

Objective To investigate the expression and possible roles of Wnt inhibitory factor-1 (Wif-1) and β-catenin in the Wnt pathway in childhood acute lymphoblastic leukemia (ALL). Methods The clinical data of 35 children who had newly-diagnosed ALL and achieved complete remission on day 33 of remission induction therapy were retrospectively reviewed. The children before treatment were considered as the incipient group, and those who achieved complete remission on day 33 were considered as the remission group. Fifteen children with non-malignant hematologic diseases were enrolled as the control group. RT-PCR was used to measure the mRNA expression of Wif-1 and β-catenin. ELISA was used to measure the protein expression of Wif-1. Results Compared with the control and remission groups, the incipient group had significantly lower mRNA and protein expression of Wif-1 and significantly higher mRNA expression of β-catenin (P < 0.05). In the incipient and remission groups, high-risk children showed significantly higher mRNA expression of β-catenin and significantly lower mRNA and protein expression of Wif-1 than the medium- and low-risk children (P < 0.05). In the incipient and remission group, the children with T-cell acute lymphoblastic leukemia showed significantly higher mRNA expression of β-catenin and significantly lower mRNA and protein expression of Wif-1 compared with those with B-lineage acute lymphoblastic leukemia (P < 0.05). In each group, there was a negative correlation between the mRNA expression of Wif-1 and β-catenin (P < 0.05). Conclusions Reduced expression of Wif-1 and increased expression of β-catenin may be involved in the pathogenesis of childhood ALL, and the degree of reduction in Wif-1 and/or increase in β-catenin may be related to prognosis.