基于化学发光法建立深圳地区健康儿童性激素的参考区间

Establishment of reference ranges of sex hormones for healthy children in Shenzhen, China based on chemiluminescence

摘要:

目的 建立深圳地区0~18岁健康儿童性激素6项，即黄体生成素、促卵泡生成素、孕酮、泌乳素、雌二醇、睾酮化学发光法的参考区间值。方法 采用分层整群抽样的方法于2015年9月至2016年9月在深圳市福田、罗湖、南山、宝安、龙岗5个区随机抽取0~18岁健康儿童2 178例（男童1 219例，女童959例），包括新生儿81例，婴儿335例，幼儿346例，学龄前儿童469例，学龄期儿童419例，青春期儿童528例。利用美国贝克曼DXI800化学发光仪测定黄体生成素、促卵泡生成素、孕酮、泌乳素、雌二醇、睾酮6项性激素水平。结果 黄体生成素、促卵泡生成素、孕酮、泌乳素、雌二醇和睾酮在不同年龄组间水平不同，差异有统计学意义（P < 0.05）。同年龄组不同性别间的性激素水平的比较差异也有统计学意义（P < 0.05）。基于各年龄组不同性别儿童的6项激素检测水平，建立了深圳地区0~18岁健康儿童性激素6项的参考区间。结论 深圳地区0~18岁健康儿童不同年龄组和不同性别组的性激素水平存在差异。该研究建立的不同性别的各年龄组的性激素6项的参考区间对儿童内分泌相关疾病的诊断与治疗有重要意义。

Abstract:

Objective To study the reference ranges of six sex hormones, i.e., luteinizing hormone, follicle-stimulating hormone, progesterone, prolactin, estradiol, and testosterone, for healthy children aged 0-18 years in Shenzhen, China. Methods Stratified cluster sampling was performed to select 2 178 healthy children aged 0-18 years in the districts of Futian, Luohu, Nanshan, Bao'an, and Longgang in Shenzhen between September 2015 and September 2016. There were 1 219 boys and 959 girls, including 81 neonates, 335 infants, 346 young children, 469 preschool children, 419 school-aged children, and 528 adolescents. The American Beckman DXI800 chemiluminescence meter was used to measure the levels of luteinizing hormone, follicle-stimulating hormone, progesterone, prolactin, estradiol, and testosterone. Results There were significant differences in the levels of luteinizing hormone, follicle-stimulating hormone, progesterone, prolactin, estradiol, and testosterone between different age groups (P < 0.05). There were also significant differences in the levels of these sex hormones between boys and girls in the same age group (P < 0.05). The reference ranges of six sex hormones were established for healthy children aged 0-18 years in Shenzhen based on the levels of these hormones in different age groups. Conclusions There are significant differences in sex hormones between different age groups or sex groups. The reference ranges of six sex hormones established for different sexes or ages have great significance in the diagnosis and treatment of endocrine diseases in children.