血清高迁移率族蛋白B1与新生儿呼吸窘迫综合征的相关性研究

Association between high-mobility group box 1 and neonatal respiratory distress syndrome

摘要:

目的 探讨血清高迁移率族蛋白B1（HMGB1）水平与新生儿呼吸窘迫综合征（NRDS）的相关性。方法 选取NRDS患儿35例（观察组）及正常新生儿35例（对照组），收集两组新生儿生后12~24 h内外周静脉血，采用酶联免疫法（ELISA）检测两组新生儿血清HMGB1水平。结果 NRDS患儿血清HMGB1水平高于对照组（P < 0.05），重度NRDS患儿血清HMGB1水平高于轻度患儿（P < 0.05），死亡的NRDS患儿血清HMGB1水平高于存活患儿（P < 0.05）。ROC曲线分析显示预测NRDS的AUC值为0.846（95% CI：0.755~0.936），血清HMGB1 625.3 pg/mL为预测NRDS的最佳界值，预测NRDS患儿死亡风险的AUC为0.916（95% CI：0.813~1.000），血清HMGB1 772.2 pg/L为预测NRDS患儿死亡的最佳界值。结论 NRDS患儿血清HMGB1水平显著升高，血清HMGB1可较好地预测NRDS的发生及预后。

Abstract:

Objective To study the association between serum level of high-mobility group box 1(HMGB1) and neonatal respiratory distress syndrome (NRDS). Methods A total of 35 infants with NRDS and 35 normal neonates (control group) were enrolled. Peripheral venous blood samples were collected with 12-24 hours after birth. ELISA was used to measure the serum level of HMGB1. Results The infants with mild and severe NRDS had a significantly higher serum level of HMGB1 than the control group (P < 0.05). The infants with severe NRDS had a significantly higher serum level of HMGB1 than those with mild NRDS (P < 0.05). The infants with NRDS who died had a significantly higher serum level of HMGB1 than those who survived (P < 0.05). The receiver operating characteristic (ROC) curve showed that the optimal cut-off value for serum level of HMGB1 to predict NRDS was 625.3 pg/mL with an area under the ROC curve (AUC) of 0.846 (95%CI:0.755-0.936), and the optimal cut-off value for serum level of HMGB1 to predict the death of infants with NRDS was 772.2 pg/mL with an AUC of 0.916 (95%CI:0.813-1.000). Conclusions Infants with NRDS have a significant increase in the serum level of HMGB1, and the serum level of HMGB1 can well predict the development and prognosis of NRDS.