RSV感染患儿呼吸道上皮细胞中干扰素-λ1的表达水平及其与RSV载量的关系

Expression of interferon-λ1 in respiratory epithelial cells of children with RSV infection and its relationship with RSV load

摘要:

目的 了解呼吸道合胞病毒（RSV）感染患儿呼吸道上皮细胞中干扰素（IFN）-λ1的表达水平及其与RSV载量的关系。方法 收集2015年6月至2016年6月期间因呼吸道感染住院患儿的鼻咽拭子标本，采用直接免疫荧光法行7种常见呼吸道病毒（含RSV）抗原检测，筛选出单纯RSV阳性患儿作为RSV感染组（n=120）；另收集同期门诊体检健康儿童鼻咽拭子标本，行7种常见呼吸道病毒检查均为阴性的儿童作为健康对照组（n=50）。对两组儿童鼻咽拭子标本行荧光定量PCR检测RSV载量和IFN-λ1 mRNA水平。结果 RSV感染组鼻咽拭子中IFN-λ1 mRNA水平显著高于健康对照组（P < 0.05），且IFN-λ1 mRNA水平与RSV载量呈正相关（r=0.56，P < 0.05）。结论 RSV可诱导呼吸道上皮细胞中IFN-λ1的表达，推测IFN-λ1在机体抗RSV感染中可能起重要作用。

Abstract:

Objective To investigate the expression of IFN-λ1 in respiratory epithelial cells of children with respiratory syncytial virus (RSV) infection and its relationship with RSV load. Methods The nasopharyngeal swabs were collected from the children who were hospitalized with respiratory tract infection from June 2015 to June 2016. A direct immunofluorescence assay was used to detect the antigens of seven common respiratory viruses (including RSV) in the nasopharyngeal swabs. A total of 120 children who were only RSV positive were selected as the RSV infection group. A total of 50 children who had negative results in the detection of all viral antigens were selected as the healthy control group. Fluorescence quantitative real-time PCR was used to determine the RSV load and the expression of IFN-λ1 mRNA in the nasopharyngeal swabs of children in the two groups. Results The expression of IFN-λ1 in the RSV infection group was significantly higher than that in the healthy control group (P < 0.05). The expression of IFN-λ1 was positively correlated with RSV load (r=0.56, P < 0.05). Conclusions RSV can induce the expression of IFN-λ1 in respiratory epithelial cells, suggesting that IFN-λ1 may play an important role in anti-RSV infection.