肠道病毒71型感染手足口病S100B基因多态性研究

Association between S100B gene polymorphisms and hand, foot and mouth disease caused by enterovirus 71 infection

摘要:

目的 研究S100B基因rs9722位点基因多态性与肠道病毒71型（EV71）感染手足口病（HFMD）的相关性。方法 以124例EV71感染HFMD患儿为研究对象，56例健康儿童作为对照组，两组均进行S100B基因rs9722位点基因多态性检测；并且74例HFMD患儿进行血清S100B蛋白检测。结果 S100B基因rs9722位点检出3种基因型：CC型、CT型和TT型，基因型频率分布符合Hardy-Weinberg平衡。与对照组比较，HFMD组患儿S100B基因rs9722位点TT基因型频率和T等位基因频率明显增高（P < 0.01）。重症EV71感染相关HFMD患儿TT基因型频率和T等位基因频率均明显高于普通型患儿（P < 0.05）。与痊愈组比较，HFMD不良预后组的S100B基因rs9722位点TT基因型频率和T等位基因频率明显增高（P < 0.05）。74例HFMD患儿各基因型的血清S100B蛋白水平以TT基因型最高，CC型最低（P < 0.01）。结论 S100B基因rs9722位点T等位基因可能是EV71感染HFMD发展为重症的危险因素。

Abstract:

Objective To investigate the association between rs9722 polymorphisms in the S100B gene and hand, foot and mouth disease (HFMD) caused by enterovirus 71.Methods A total of 124 HFMD children with enterovirus 71 infection were enrolled as subjects, and 56 healthy children were enrolled as control group. The rs9722 polymorphisms in the S100B gene were detected for both groups, and the serum level of S100B protein was measured for 74 HFMD children.Results The rs9722 locus of the S100B gene had three genotypes, CC, CT, and TT, and the genotype frequencies were in accordance with Hardy-Weinberg equilibrium. Compared with the control group, the HFMD group had significant increases in the frequencies of TT genotype and T allele (P < 0.01). Children with severe HFMD caused by enterovirus 71 infection had significantly higher frequencies of TT genotype and T allele than those with moderate or mild HFMD (P < 0.05). Compared with the cured patients, the patients with poor prognosis had significant increases in the frequencies of TT genotype and T allele in the rs9722 locus of the S100B gene (P < 0.05). Among the 74 children with HFMD, the children with TT genotype had the highest serum level of S100B protein, and those with CC genotype had the lowest level (P < 0.01).Conclusions T allele in the rs9722 locus of the S100B gene might be a risk factor for severe HFMD caused by enterovirus 71 infection.