人巨细胞病毒感染婴儿γδT、Treg细胞和相关细胞因子的表达及临床意义

Percentages of peripheral blood γδ T cells and regulatory T cells and expression of associated cytokines in infants with human cytomegalovirus infection

摘要:

目的 探讨人巨细胞病毒（HCMV）感染婴儿外周血γδT细胞、调节性T细胞（Treg）的表达和相关细胞因子的含量及其临床意义。方法 以22例HCMV感染婴儿（HCMV感染组）和22例门诊健康体检婴儿（对照组）为研究对象，流式细胞术检测两组外周血γδT细胞和Treg细胞的表达，ELISA法检测两组血浆IL-17、TGF-β1水平。结果 HCMV感染组的γδT细胞表达和IL-17水平高于对照组（P < 0.01）；Treg细胞表达和TGF-β1水平低于对照组（P < 0.01）。HCMV组的γδT细胞表达与Treg细胞表达、TGF-β1水平呈负相关（P < 0.05），而与IL-17水平呈正相关（P < 0.05）；Treg细胞表达与TGF-β1水平呈正相关而与IL-17水平呈负相关（P < 0.05），IL-17水平与TGF-β1水平无相关性（P > 0.05）。结论 HCMV感染婴儿存在γδT细胞和Treg细胞表达的失衡，γδT细胞可能参与了IL-17的分泌。

Abstract:

Objective To investigate the percentages of peripheral blood γδ T cells and regulatory T cells (Treg) and the expression of associated cytokines, interleukin 17 (IL-17) and transforming growth factor-β1 (TGF-β1), in infants with human cytomegalovirus (HCMV) infection. Methods Twenty-two infants with HCMV infection (HCMV group) and 22 healthy infants who underwent physical examination (control group) were enrolled in this study. The percentages of peripheral blood γδ T cells and Treg cells were determined by flow cytometry. The levels of IL-17 and TGF-β1 in plasma were measured using ELISA. Results Compared with the control group, the HCMV group had significantly higher percentage of γδ T cells and IL-17 level (P < 0.01) and significantly lower percentage of Treg cells and TGF-β1 level (P < 0.01). In the HCMV group, the percentage of γδ T cells was negatively correlated with the percentage of Treg cells and TGF-β1 level (P < 0.05), but positively correlated with IL-17 level (P < 0.05); the percentage of Treg cells was positively correlated with TGF-β1 level (P < 0.05), but negatively correlated with IL-17 level (P < 0.05); there was no correlation between IL-17 level and TGF-β1 level (P > 0.05). Conclusions There is an imbalance between γδ T cells and Treg cells in the peripheral blood of infants with HCMV infection, and γδ T cells may be involved in the secretion of IL-17.