抽动障碍儿童Th淋巴细胞及其亚群的变化

Changes in T helper lymphocytes and their subsets in children with tic disorders

摘要:

目的 探讨抽动障碍（TD）儿童T辅助（Th）淋巴细胞及其亚群表达变化的临床意义。方法 采用流式细胞技术检测TD组和对照组（健康儿童）外周血中Th淋巴细胞及其亚群的表达水平。结果 与对照组比较，TD组Th淋巴细胞的百分比明显降低，差异有统计学意义（P < 0.001）；Th淋巴细胞的异常率为68.7%，比对照组（18.8%）增高（P < 0.001）；而且Th淋巴细胞百分比和耶鲁综合抽动严重程度评分呈负相关（r=-0.3945，P < 0.001）。在Th淋巴细胞亚群中，TD组Th1淋巴细胞百分比明显高于对照组，而Th2淋巴细胞百分比明显低于对照组，差异均有统计学意义（P < 0.001）。结论 Th淋巴细胞及其亚群的平衡失调导致的细胞免疫功能异常可能与儿童TD的发生相关，且Th淋巴细胞水平可作为评估TD严重程度的指标。

Abstract:

Objective To explore the changes in T helper lymphocytes and their subsets in children with tic disorders (TD) and their clinical signifcance.Methods Flow cytometry was used to measure the percentages of T helper lymphocytes and their subsets in the peripheral blood of children with TD and healthy children (controls).Results The percentage of T helper lymphocytes was signifcantly lower in the TD group than in the control group (P < 0.001). The abnormal rate of T helper lymphocytes in the TD group was signifcantly higher than that in the control group (68.7% vs 18.8%; P < 0.001). The percentage of T helper lymphocytes was negatively correlated with Yale Global Tic Severity Scale score (r=-0.3945, P < 0.001). As for the subsets of T helper lymphocytes, the TD group had a signifcantly higher percentage of Th1 cells and a signifcantly lower percentage of Th2 cells compared with the control group (P < 0.001).Conclusions The abnormality of T helper lymphocytes and the imbalance of their subsets may be associated with the pathogenesis of TD in children. The percentage of T helper lymphocytes can be used as an indicator for assessing the severity of TD.