HCMV感染患儿HLA-DR、CD4+CD25+调节性T细胞及IL-17、IL-27表达水平与肝损害的相关性研究

Relationship between expression of peripheral blood HLA-DR, CD4+CD25+ regulatory T cells, IL-17 and IL-27 with liver damage in children with human cytomegalovrius infection

摘要:

目的 探讨人巨细胞病毒（HCMV）感染患儿HLA-DR、CD4⁺CD25⁺调节性T细胞（CD4⁺CD25⁺Treg）以及IL-17、IL-27的表达水平与肝功能损害的相关性。方法 以21例HCMV感染肝功能损害患儿和21例HCMV感染非肝功能损害患儿为研究对象。流式细胞术检测两组外周血CD4⁺CD25⁺Treg、HLA-DR细胞的表达；ELISA检测血浆IL-17及IL-27表达。结果 与非肝损组比较，肝损组患儿CD4⁺CD25⁺Treg比率较低，IL-17、IL-27表达水平较高，差异均有统计学意义（P < 0.05）。IL-17及IL-27水平与CD4⁺CD25⁺Treg细胞比率呈负相关（P < 0.01）。结论 CD4⁺CD25⁺Treg细胞介导的免疫耐受失衡及IL-17、IL-27等炎症因子过度激活可能参与HCMV感染肝功能损害发生。

Abstract:

Objective To study the relationship between the expression of peripheral blood HLA-DR, CD4⁺ CD25⁺ regulatory T cells, IL-17 and IL-27 with liver damage in children with human cytomegalovirus (HCMV) infection.Methods Twenty-one HCMV children with liver damage and twenty-one HCMV children without liver damage were enrolled in this study. The expression of peripheral blood HLA-DR and CD4⁺ CD25⁺ regulatory T cells was detected by flow cytometry. Plasma levels of IL-17 and IL-27 were measured using ELISA.Results The plasma levels of IL-17 and IL-27 in children with liver damage were significantly higher than in those without liver damage, while the expression of peripheral blood CD4⁺ CD25⁺ regulatory T cells was lower than in those without liver damage (P < 0.05). Plasma IL-17 and IL-27 levels were negatively correlated with the expression of peripheral blood CD4⁺ CD25⁺ regulatory T cells (P < 0.01).Conclusions Immune imbalance mediated by CD4⁺ CD25⁺ regulatory T cells and over-expression of IL-17 and IL-27 may be involved in the pathogenesis of liver damage in children with HCMV infection.