runt相关转录因子3在毛细支气管炎患儿中的表达及临床意义

Expression and clinical significance of runt-related transcription factor 3 in children with bronchiolitis

摘要:

目的 研究毛细支气管炎患儿runt相关转录因子3（RUNX3） mRNA水平，评估其在毛细支气管炎中的临床意义。方法 选取54例毛细支气管炎患儿为毛细支气管炎组，其中有特应性体质患儿28例为特应性毛细支气管炎组，非特应性体质患儿26例为非特应性毛细支气管炎组；另选取健康婴幼儿48例为健康对照组，其中有特应性体质婴幼儿24例为特应性健康对照组，非特应性体质婴幼儿24例为非特应性健康对照组。荧光定量PCR法测定外周血单个核细胞中RUNX3 mRNA水平，ELISA法检测血清IL-4和IFN-γ浓度。结果 毛细支气管炎组RUNX3 mRNA水平显著低于健康对照组，其中特应性毛细支气管炎组RUNX3 mRNA水平显著低于非特应性毛细支气管炎组、特应性健康对照组和非特应性健康对照组（P < 0.05）；毛细支气管炎组血清IL-4浓度显著高于健康对照组，其中特应性毛细支气管炎组显著高于非特应性健康对照组（P < 0.05）；毛细支气管炎组血清IFN-γ浓度显著低于健康对照组，其中特应性毛细支气管炎组显著低于非特应性毛细支气管炎组、特应性健康对照组和非特应性健康对照组（P < 0.05）；相关性分析显示RUNX3 mRNA水平与血清IL-4浓度呈负相关，与血清IFN-γ浓度呈正相关（P < 0.05）。结论 外周血单个核细胞中RUNX3基因表达量的检测对甄别毛细支气管炎中有特应性体质的哮喘高危儿具有一定临床价值。

Abstract:

Objective To study the mRNA level of runt-related transcription factor 3 (RUNX3) in children with bronchiolitis and its clinical significance in bronchiolitis. Methods A total of 54 young children with bronchiolitis were enrolled as the bronchiolitis group, among whom 28 with atopic constitution were enrolled in the atopic bronchiolitis group and 26 with non-atopic constitution were enrolled in the non-atopic bronchiolitis group. A total of 48 healthy young children were enrolled as the healthy control group, among whom 24 with atopic constitution were enrolled in the atopic healthy control group and 24 with non-atopic constitution were enrolled in the non-atopic healthy control group. Quantitative real-time PCR was used to measure the mRNA level of RUNX3 in peripheral blood mononuclear cells. ELISA was used to measure the serum levels of interleukin-4 (IL-4) and interferon gamma (IFN-γ). Results The bronchiolitis group had a significantly lower mRNA level of RUNX3 than the healthy control group, and the atopic bronchiolitis group had a significantly lower mRNA level of RUNX3 than the non-atopic bronchiolitis, atopic healthy control, and non-atopic healthy control groups (P < 0.05). The bronchiolitis group had a significantly higher serum level of IL-4 than the healthy control group, and the atopic bronchiolitis group had a significantly higher serum level of IL-4 than the non-atopic healthy control group (P < 0.05). The bronchiolitis group had a significantly lower serum level of IFN-γ than the healthy control group, and the atopic bronchiolitis group had a significantly lower serum level of IFN-γ than the non-atopic bronchiolitis, atopic healthy control, and non-atopic healthy control groups (P < 0.05). The correlation analysis showed that the mRNA level of RUNX3 was negatively correlated with the serum level of IL-4 and was positively correlated with the serum level of IFN-γ (P < 0.05). Conclusions Measurement of RUNX3 gene expression in peripheral blood mononuclear cells has a certain value in identifying children with atopic constitution at high risk of asthma among children with bronchiolitis.