干扰素-λ1在人类鼻病毒感染后儿童呼吸道上皮细胞中的表达

Expression of interferon-λ1 in respiratory epithelial cells in children with human rhinovirus infection

摘要:

目的 研究人类鼻病毒（HRV）感染后儿童呼吸道上皮细胞中干扰素（IFN）-λ1的表达水平。方法 收集2017年2~10月因急性呼吸道感染住院的患儿痰液及鼻咽拭子标本，分别进行细菌培养及11种呼吸道病原体核酸检测，筛检出单纯HRV阳性患儿90例作为HRV感染组，单纯呼吸道合胞病毒（RSV）阳性患儿95例为RSV感染组。另选取同期门诊体检且病原检测结果均阴性的健康儿童50例为健康对照组。采集各组儿童鼻咽拭子标本，采用荧光定量PCR检测病毒载量和IFN-λ1 mRNA表达水平。结果 在HRV感染组，IFN-λ1 mRNA的表达水平在不同性别及不同年龄组间比较差异均无统计学意义（P > 0.05）。HRV感染组IFN-λ1 mRNA的表达水平与HRV的病毒载量无相关性（P > 0.05）。HRV感染组的IFN-λ1 mRNA表达水平高于健康对照组，但低于RSV感染组（P < 0.05）。结论 HRV可诱导呼吸道上皮细胞中IFN-λ1的表达；推测IFN-λ1在机体抗HRV感染中可能起到重要作用。

Abstract:

Objective To study the expression of interferon-λ1 (IFN-λ1) in respiratory epithelial cells in children with human rhinovirus (HRV) infection. Methods Sputum samples and nasopharyngeal swabs were collected from the children who were hospitalized due to acute respiratory infection from February to October, 2017. Bacterial culture was performed, and nucleic acid test was performed for 11 respiratory pathogens. A total of 90 children with positive HRV alone were enrolled as the HRV infection group, and 95 children with positive respiratory syncytial virus (RSV) alone were enrolled as the RSV infection group. A total of 50 healthy children who underwent outpatient physical examination during the same period of time and had negative results for all pathogen tests were enrolled as the healthy control group. Nasopharyngeal swabs were collected from all groups, and quantitative real-time PCR was used to measure viral load and the mRNA expression of IFN-λ1. Results In the HRV infection group, there was no significant difference in the mRNA expression of IFN-λ1 between boys and girls and across all age groups (P > 0.05). In the HRV infection group, there was no correlation between the mRNA expression of IFN-λ1 and HRV load (P > 0.05). The mRNA expression of IFN-λ1 in the HRV infection group was significantly higher than that in the healthy control group (P < 0.05), but significantly lower than that in the RSV infection group (P < 0.05). Conclusions HRV can induce the expression of IFN-λ1 in respiratory epithelial cells, suggesting that IFN-λ1 may play an important role in anti-HRV infection in children.