原癌基因Fra-1在肾母细胞瘤患儿血液中的表达及其意义

Expression of the Fra-1 gene in the peripheral blood of children with Wilms tumor

摘要:

目的 探讨原癌基因Fra-1在肾母细胞瘤患儿血液中的表达及其意义。方法 选取2012年12月至2018年1月病理明确诊断为肾母细胞瘤的患儿作为病例组（n=50），健康体检儿童作为对照组（n=40）；获得随访的45例患儿中，将持续缓解患儿纳入理想疗效组（n=33），复发、转移或死亡患儿纳入疗效不佳组（n=12）。采集所有研究对象外周血，通过实时荧光定量PCR检测Fra-1 mRNA表达水平。结果 肾母细胞瘤患儿血液中Fra-1 mRNA相对表达量明显高于对照组（P < 0.05）。Fra-1 mRNA的表达水平在肾母细胞瘤是否远处转移及TNM分期间比较差异有统计学意义（P < 0.05），而在患儿的性别、年龄、肿瘤直径、肿瘤位置及不同甲胎蛋白（AFP）水平间比较差异均无统计学意义（P > 0.05）。理想疗效组Fra-1 mRNA相对表达量低于疗效不佳组（P < 0.05）。结论 Fra-1可能参与肾母细胞瘤的形成，并在其发展、侵袭及转移中起一定作用，但具体机制仍有待深入研究。

Abstract:

Objective To study the expression of the Fra-1 gene in the peripheral blood of children with Wilms tumor and its clinical significance. Methods Fifty children pathologically diagnosed with Wilms tumor between December 2012 and January 2018 were enrolled as the case group, and 40 healthy children for physical examination were selected as the control group. Among the 45 children with Wilms tumor who were followed up, the children with continuous remission were included in the ideal efficacy group (n=33), and those with recurrence, metastasis or death were included in the poor efficacy group (n=12). Peripheral blood samples were collected from all subjects. Quantitative real-time PCR was used to measure the mRNA expression of Fra-1. Results The case group had significantly higher mRNA expression of Fra-1 in peripheral blood than the control group (P < 0.05). In the case group, Fra-1 mRNA expression was significantly different between the individuals with and without distant metastasis and those with different TNM stages (P < 0.05), but was not significantly different between the individuals with different sexes, ages, tumor diabetes, tumor locations and alpha-fetoprotein levels (P > 0.05). The mRNA expression of Fra-1 was significantly lower in the ideal efficacy group than in the poor efficacy group (P < 0.05). Conclusions Fra-1 may be involved in the development of Wilms tumor and plays a certain role in its development, invasion and metastasis, but the mechanism remains to be further studied.