Wnt通路中散乱蛋白在儿童急性淋巴细胞白血病中的表达及意义

Expression and significance of dishevelled proteins in the Wnt pathway in childhood acute lymphoblastic leukemia

摘要:

目的 探讨Wnt信号通路中散乱蛋白（DVL）对儿童急性淋巴细胞白血病（ALL）发病及预后的意义。方法 选取33例新发ALL患儿为病例组，根据危险度将ALL患儿分为低危组（n=14）、中危组（n=5）和高危组（n=14）；29例免疫性血小板减少症（ITP）患儿为对照组。采集病例组患儿初发及诱导治疗第33天时，以及对照组骨髓血各2 mL，采用实时荧光定量聚合酶链反应（qRT-PCR）检测骨髓血细胞DVL1、DVL2、DVL3 mRNA表达。结果 病例组初发期DVL1、DVL2、DVL3 mRNA表达水平高于缓解期及对照组（P < 0.05），与对照组比较，病例组缓解期DVL2 mRNA水平升高（P < 0.05）。DVL2 mRNA表达水平在病例组初发期及缓解期均高于DVL 1、DVL 3 mRNA （P < 0.05）。高、中危组DVL1、DVL2 mRNA表达水平高于低危组（P < 0.05）。DVL2 mRNA表达水平在低、中、高危组均高于DVL1、DVL3 mRNA （P < 0.05）。结论 Wnt通路中DVL，尤其是DVL2的表达变化可能对儿童ALL的发病及预后有一定意义。

Abstract:

Objective To study the significance of dishevelled (DVL) proteins in the Wnt signaling pathway in the pathogenesis and prognosis of childhood acute lymphoblastic leukemia (ALL). Methods A total of 33 children with new-onset ALL were enrolled as the case group. According to the degree of risk, they were divided into 3 groups:low-risk (n=14), intermediate-risk (n=5) and high-risk (n=14). A total of 29 children with immune thrombocytopenia were enrolled as the control group. At diagnosis and on day 33 of induction therapy, 2 mL bone marrow samples were collected from the case and control groups, and qRT-PCR was used to measure the mRNA expression of DVL1, DVL2 and DVL3 in blood cells of bone marrow. Results The mRNA expression of DVL1, DVL2 and DVL3 in the case group in the incipient stage was significantly higher than that in the remission stage and the control group (P < 0.05). Compared with the control group, the case group had a significant increase in the mRNA expression of DVL2 in the remission stage (P < 0.05). The mRNA expression of DVL2 was significantly higher than that of DVL1 and DVL3 in both remission and incipient stages (P < 0.05). The high-and intermediate-risk groups had significantly higher mRNA expression of DVL1 and DVL2 than the low-risk group (P < 0.05). The mRNA expression of DVL2 was significantly higher than that of DVL1 and DVL3 in the low-, intermediate-and high-risk groups (P < 0.05). Conclusions The change in the expression of DVL, especially DVL2, in the Wnt signal pathway has certain significance in the pathogenesis and prognosis of childhood ALL.