白细胞介素-10基因多态性与儿童肠道病毒71型感染的相关性研究

Association of interleukin-10 gene polymorphism with enterovirus 71 infection in children

摘要:

目的 探讨白细胞介素-10（IL-10）基因位点-1082A/G、-819C/T和-592C/A多态性与IL-10水平及儿童肠道病毒71型（EV71）感染程度的关系。方法 选取EV71感染的手足口病患儿137例为研究对象，其中轻症组91例，重症组46例，另选取行健康体检儿童122例为健康对照组，采集临床数据。采用酶联免疫吸附试验（ELISA）检测血清IL-10水平。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析技术检测IL-10基因位点-1082A/G、-819C/T和-592C/A的多态性。结果 与健康对照组相比，EV71感染组患儿的-1082位点AA基因型频率和A等位基因频率更高（P < 0.05）。在EV71感染组中，重症组-1082位点AA基因型频率和A等位基因频率高于轻症组（P < 0.05）。两组间IL-10基因位点-819C/T和-592C/A多态性的分布比较差异均无统计学意义（P > 0.05）。重症组患儿血清IL-10水平明显高于轻症组和健康对照组（P < 0.05）。IL-10-1082 AA基因型、-819 TT基因型和-592 AA基因型与IL-10的低表达有关（P < 0.05）。在单倍型构建上，EV71感染组GCC单倍型的频率低于健康对照组（P < 0.05）。在EV71重症感染组中，ATA单倍型患儿的IL-10水平较其他单倍型显著降低，而GCC患儿的IL-10水平较其他单倍型显著升高（P < 0.05）；轻症组和健康对照组各单倍型间的IL-10水平比较差异无统计学意义（P > 0.05）。结论 IL-10基因多态性与IL-10表达水平及EV71感染严重程度有关。

Abstract:

Objective To study the association of interleukin-10 (IL-10) -1082A/G, -819C/T, and -592C/A polymorphisms with IL-10 level and the severity of enterovirus 71 (EV71) infection in children. Methods A total of 137 children with hand-foot-mouth disease due to EV71 infection were enrolled as EV71 infection group, which was further divided into mild group with 91 children and severe group with 46 children, and 122 healthy children who underwent physical examination were enrolled as healthy control group. Related clinical data were collected. ELISA was used to measure the serum level of IL-10, and polymerase chain reaction-restriction fragment length polymorphism was used to analyze IL-10 -1082A/G, -819C/T and -592C/A polymorphisms. Results Compared with the healthy control group, the children with EV71 infection had significantly higher frequency of -1082 AA genotype and A allele (P < 0.05). Among the children with EV71 infection, the severe group had significantly higher frequency of -1082 AA genotype and A allele than the mild group (P < 0.05), while there was no significant difference in the distribution of IL-10 -819C/T and IL-10 -592C/A polymorphisthan the mild group and the healthy control group. IL-10 -1082 AA genotype, -819 TT genotype, and -592 AA genotype were asms between the two groups (P > 0.05). The severe group had a significantly higher serum level of IL-10 sociated with the low expression of IL-10 (P < 0.05). As for haplotype, the EV71 infection group had a significantly lower frequency of GCC haplotype than the healthy control group (P < 0.05). In the severe group, the children with ATA haplotype had a significantly lower IL-10 level than those with other haplotypes, and the children with GCC haplotype had a significantly higher IL-10 level than those with other haplotypes (P < 0.05). There was no significant difference in IL-10 level between children with different haplotypes in the mild group and the healthy control group (P > 0.05). Conclusions IL-10 gene polymorphisms are associated with IL-10 expression and the severity of EV71 infection in children.