血清microRNA-122与肥胖儿童胰岛素抵抗的关系

Correlation between serum microRNA-122 and insulin resistance in obese children

摘要:

目的 研究肥胖儿童血清microRNA-122（miR-122）与胰岛素抵抗的关系。方法 选取47例7~14岁重度肥胖儿童为肥胖组，另选取与肥胖组性别及年龄匹配的正常体重健康儿童45例作为健康对照组，分别检测并记录两组儿童的身高、体重、腰围、臀围、空腹血糖（FBG）、空腹胰岛素（FINS）、甘油三酯（TG）、总胆固醇（TC）、游离脂肪酸（FFA）、白介素-6（IL-6）、miR-122水平，计算体重指数（BMI）、腰臀比（WHR）、胰岛素抵抗指数（HOMA-IR），并进行统计分析。结果 与健康对照组相比，肥胖组儿童身高、体重、BMI、WHR及FINS、HOMA-IR、TG、FFA、IL-6、miR-122水平均升高（P < 0.05）；肥胖组miR-122水平与FINS、HOMA-IR、IL-6水平呈正相关（分别r=0.408、0.442、0.464，P < 0.05）；miR-122的变化与IL-6有线性回归关系，且呈正相关（b'=0.318，P < 0.05）。结论 肥胖儿童血清miR-122可能与胰岛素抵抗相关，具体机制尚需进一步研究。

Abstract:

Objective To study the relationship between serum microRNA-122 (miR-122) and insulin resistance in obese children. Methods Forty-seven children with severely obesity aged 7-14 years and 45 age-and gender matched healthy children with normal weight (control group) were enrolled. The levels of height, weight, waistline, hip circumference, fasting blood glucose (FBG), fasting insulin (FINS), triglyceride (TG), total cholesterol (TC), free fatty acid (FFA), interleukin-6 (IL-6) and miR-122 in the two groups were measured. Body mass index (BMI), waist-hip ratio (WHR) and insulin resistance index (HOMA-IR) were calculated. Results Compared with the control group, the height, weight, BMI, WHR, FINS, HOMA-IR, TG, FFA, IL-6, and miR-122 levels in the obese group were significantly increased (P < 0.05). MiR-122 levels in the obese group were positively correlated with FINS, HOMA-IR and IL-6 levels (r=0.408, 0.442, and 0.464 respectively, P < 0.05). The changes of miR-122 have a linear regression relationship with IL-6 (b'=0.318, P < 0.05). Conclusions The elevated serum miR-122 levels may be correlated with insulin resistance in obese children. The mechanism needs to be further studied.