IL-8基因rs4073位点多态性与新生儿败血症易感性的关系

Association between interleukin-8 rs4073 polymorphisms and susceptibility to neonatal sepsis

摘要:

目的 采用前瞻性研究方法探讨白细胞介素-8（IL-8）基因rs4073位点多态性与足月新生儿败血症易感性的关系。方法 选取2017年1~12月通过血培养阳性确诊为败血症的新生儿50例为败血症组；有临床症状但血培养阴性的50例新生儿为临床败血症组；另选取同期50例非感染新生儿作为对照组。采用测序技术检测IL-8基因rs4073位点多态性，比较3组间基因型和等位基因频率的分布差异；采用多因素logistic回归分析IL-8基因rs4073位点基因型与足月新生儿败血症易感性的关系。结果 IL-8基因rs4073位点基因型及等位基因频率的分布在3组间比较差异均有统计学意义（P < 0.05）。logistic回归分析显示低胎龄和IL-8基因rs4073位点TT基因型可能是新生儿败血症发病的危险因素（P < 0.05）。结论 IL-8基因rs4073位点TT基因型可能与足月新生儿败血症易感有关。

Abstract:

Objective To study the association between interleukin-8 (IL-8) rs4073 polymorphisms and susceptibility to sepsis in full-term neonates through a prospective study. Methods A total of 50 neonates who were diagnosed with sepsis based on positive blood culture from January to December 2017 were enrolled as the sepsis group. Fifty neonates who had clinical symptoms and negative blood culture were enrolled as the clinical sepsis group. Fifty neonates without infection were enrolled as the control group. Sequencing was used to detect the polymorphisms of IL-8 rs4073. The three groups were compared in terms of the frequencies of genotypes and alleles. A multivariate logistic regression analysis was used to investigate the association of IL-8 rs4073 genotypes with sepsis in full-term neonates. Results There were significant differences in the frequencies of genotypes and alleles at IL-8 rs4073 among the three groups (P < 0.05). The logistic regression analysis showed that a low gestational age and TT genotype at IL-8 rs4073 were risk factors for the pathogenesis of sepsis in neonates (P < 0.05). Conclusions The full-term neonates with TT genotype at IL-8 rs4073 may be susceptible to sepsis.