支气管肺发育不良早产儿血清YKL-40和HMGB1水平的变化

Changes in serum human cartilage glycoprotein-39 and high-mobility group box 1 in preterm infants with bronchopulmonary dysplasia

摘要:

目的 探究早产儿生后外周血人软骨糖蛋白-39（YKL-40）、高迁移率族蛋白1（HMGB1）水平动态变化与支气管肺发育不良（BPD）的关系。方法 前瞻性选择2017年7月至2019年8月新生儿重症监护室收治的出生胎龄≥ 28周且<32周、出生体重<1 500 g的早产儿，根据诊断分为BPD组（n=35）和非BPD组（n=51）。通过酶联免疫吸附法检测早产儿生后第3、7、14天血清YKL-40和HMGB1浓度并进行比较。结果 BPD组第3、7、14天血清YKL-40浓度均低于非BPD组（P < 0.001），HMGB1浓度均高于非BPD组（P < 0.001）。两组第7、14天血清YKL-40及HMGB1浓度均高于第3天（P < 0.017）。BPD组第14天HMGB1浓度高于第7天（P < 0.017），第7、14天YKL-40浓度差异无统计学意义（P > 0.017）。非BPD组第14天YKL-40浓度高于第7天（P < 0.017），第7、14天HMGB1浓度差异无统计学意义（P > 0.017）。结论 BPD及非BPD早产儿生后第3、7、14天外周血中YKL-40及HMGB1水平存在差异，两者可能与BPD的形成相关。

Abstract:

Objective To study the association of the dynamic changes of peripheral blood human cartilage glycoprotein-39 (YKL-40) and high-mobility group box 1 (HMGB1) with bronchopulmonary dysplasia (BPD) in preterm infants. Methods Preterm infants, with a gestational age of 28-32 weeks and a birth weight of <1 500 g, who were admitted to the neonatal intensive care unit from July 2017 to August 2019 were prospectively selected and divided into a BPD group with 35 infants and a non-BPD group with 51 infants. ELISA was used to measure the serum concentrations of YKL-40 and HMGB1 in preterm infants on days 3, 7, and 14 after birth. Results The BPD group had a significantly lower serum YKL-40 concentration and a significantly higher serum HMGB1 concentration than the nonBPD group on days 3, 7, and 14 (P < 0.001). The serum concentrations of YKL-40 and HMGB1 on days 7 and 14 were significantly higher than those on day 3 in both groups (P < 0.017). In the BPD group, HMGB1 concentration on day 14 was significantly higher than that on day 7 (P < 0.017), while there was no significant change in YKL-40 concentration from day 7 to day 14 (P > 0.017). In the non-BPD group, YKL-40 concentration on day 14 was significantly higher than that on day 7 (P < 0.017), while there was no significant change in HMGB1 concentration from day 7 to day 14 (P > 0.017). Conclusions There are significant differences in the levels of YKL-40 and HMGB1 in peripheral blood between the preterm infants with BPD and those without BPD on days 3, 7, and 14 after birth, suggesting that YKL-40 and HMGB1 might be associated with the development of BPD.