宏基因组二代测序在疑难感染性疾病中的临床应用价值

Clinical value of metagenomic next-generation sequencing in complicated infectiousdiseases

摘要:

感染性疾病是临床常见疾病，病原体诊断是其诊治中的关键环节。而常规病原体检测方法耗时长、阳性率低，难以满足临床需求，宏基因组二代测序（metagenomic next-generation sequencing，mNGS）作为一种新型病原检测方法，检测范围广泛，可检测细菌、病毒、真菌、寄生虫、罕见病原体、甚至未知病原体。mNGS具有无偏倚性，可以快速、高效、准确获得检测样本中所有核酸信息，分析出致病病原体，指导临床诊断和治疗，在疑难感染性疾病中发挥着重要作用。该文综述mNGS在细菌、真菌、病毒、寄生虫等感染中的诊断优势和临床应用价值。

Abstract:

Infectious diseases are commonly seen in clinical practice, and pathogen diagnosis is the key link in diagnosis and treatment; however, conventional pathogen detection methods cannot meet clinical needs due to time-consuming operation and low positive rate. As a new pathogen detection method, metagenomic next-generation sequencing (mNGS) has a wide detection range and can detect bacteria, viruses, fungi, parasites, rare pathogens, and even unknown pathogens. The technique of mNGS is unbiased and can rapidly, efficiently, and accurately obtain all nucleic acid information in test samples, analyze pathogens, and guide clinical diagnosis and treatment, thereby playing an important role in complicated infectious diseases. This article reviews the diagnostic advantages and clinical value of mNGS in bacterial, fungal, viral, and parasitic infections.