不同浓度阿达帕林诱导SH-SY5Y细胞的分化及凋亡

Different concentrations of adapalene induce differentiation and apoptosis of SH-SY5Y cells

摘要:

目的 探讨不同浓度阿达帕林对人神经母细胞瘤细胞系SH-SY5Y细胞形态学及功能的影响，以及诱导细胞分化及凋亡的作用。方法 将SH-SY5Y细胞分为对照组、低浓度（0.1 μM和1 μM）阿达帕林组、高浓度（10 μM）阿达帕林组。采用延时显微拍摄技术观察SH-SY5Y细胞形态学变化，采用免疫荧光染色法检测神经元特异性标志物β-微管蛋白Ⅲ和成熟神经元标志物神经丝重链多肽表达，采用多电极阵列记录SH-SY5Y细胞的电生理特征，细胞凋亡检测试剂盒检测细胞凋亡情况。结果 低浓度阿达帕林促进SH-SY5Y细胞突起形成，突起之间相互连接形成网络；低浓度阿达帕林处理SH-SY5Y细胞可见自发性放电活动。与对照组比较，1 μM阿达帕林组SH-SY5Y细胞β-微管蛋白Ⅲ和神经丝重链多肽表达升高，高浓度阿达帕林组细胞凋亡水平升高（P<0.05）。结论 低浓度阿达帕林可诱导SH-SY5Y细胞分化为成熟的功能性神经元，高浓度阿达帕林可诱导SH-SY5Y细胞凋亡。

Abstract:

Objective To investigate the effects of different concentrations of adapalene on the morphology and functions of neuroblastoma cell line SH-SY5Y, as well as its role in inducing cell differentiation and apoptosis.Methods SH-SY5Y cells were divided into control group, low concentration (0.1 μM and 1 μM) adapalene groups, and high concentration (10 μM) adapalene group. Time-lapse microscopy was used to observe the morphological changes of SH-SY5Y cells. Immunofluorescence staining was performed to detect the expression of neuronal specific marker βIII-tubulin and mature neuronal marker neurofilament heavy polypeptide (NFH). Multi-electrode array was used to record the electrophysiological features of SH-SY5Y cells. Cell apoptosis was evaluated using a cell apoptosis detection kit.Results Low concentrations of adapalene promoted the formation of neurite outgrowth in SH-SY5Y cells, with the neurites interconnected to form a network. Spontaneous discharge activity was observed in SH-SY5Y cells treated with low concentrations of adapalene. Compared to the control group, the expression of βIII-tubulin and NFH increased in the 1 μM adapalene group, while the level of cell apoptosis increased in the high concentration adapalene group (P<0.05).Conclusions Low concentrations of adapalene can induce differentiation of SH-SY5Y cells into mature functional neurons, while high concentrations of adapalene can induce apoptosis in SH-SY5Y cells.