非自杀性自伤青少年COMT基因多态性与童年期创伤的交互作用

Interaction between COMT gene polymorphisms and childhood trauma in adolescents with non-suicidal self-injury

摘要:

目的 探索儿茶酚-O-甲基转移酶（catechol-O-methyltransferase, COMT）基因多态性与童年期创伤的交互作用对青少年非自杀性自伤（non-suicidal self-injury, NSSI）的影响，从而为预防和干预青少年NSSI提供依据。方法 共纳入84例NSSI青少年和87例健康对照者，收取口腔唾液对COMT基因多态性rs4680和rs165599进行基因分型。使用儿童期创伤问卷、青少年NSSI行为功能评估量表、9项患者健康问卷进行心理评估。采用层次线性回归分析，检验COMT基因多态性（rs4680、rs165599）与童年期创伤对NSSI的主效应和交互效应。利用约翰逊-内曼技术研究调节变量具有显著影响的区域。结果 COMT基因多态性rs165599与童年期创伤中情感忽视亚型的交互作用可预测青少年NSSI（β=0.251，t=2.329，P=0.022）。携带rs165599多态性G/G型的NSSI青少年，高情感忽视组的NSSI得分显著高于低情感忽视组（F=4.579，P=0.049）。结论 COMT基因多态性rs165599为G/G基因型的青少年，在遭遇童年期高情感忽视时可能会增加NSSI的易感性。

Abstract:

Objective To investigate the interaction between catechol-O-methyltransferase (COMT) gene polymorphisms and childhood trauma in adolescents with non-suicidal self-injury (NSSI), and to provide a basis for the prevention and intervention of NSSI among adolescents.Methods A total of 84 adolescents with NSSI and 87 healthy controls were enrolled in this study. Oral saliva samples were collected for genotyping of the COMT gene at rs4680 and rs165599. Childhood Trauma Questionnaire, Behavioral Function Assessment Scale of Non-suicidal Self-injury in Adolescents, and Patient Health Questionnaire-9 Items were used for mental health assessment. A hierarchical linear regression analysis was used to examine the main effect and interactive effect of COMT gene polymorphisms at rs4680 and rs165599 and childhood trauma on NSSI. The Johnson-Neyman technique was used to identify the regions where the moderating variables had a significant impact.Results The interaction between COMT gene polymorphisms at rs165599 and the subtype of emotional neglect in childhood trauma could predict NSSI in adolescents (β=0.251, t=2.329, P=0.022). As for the adolescents carrying the G/G genotype at rs165599, the high emotional neglect group had a significantly higher NSSI score than the low emotional neglect group (F=4.579, P=0.049).Conclusions Adolescents carrying the G/G genotype at rs165599 of the COMT gene may have an increase in susceptibility to NSSI in case of high emotional neglect in childhood.