GYPC和TRIP3基因表达水平与儿童急性淋巴细胞白血病预后的关系

Relationship between expression of GYPC and TRIP3 genes and prognosis of acute lymphoblastic leukemia in children

摘要:

目的研究儿童急性淋巴细胞白血病（ALL）复发及缓解的分子生物学机制，探讨GYPC、TRIP3基因的表达水平与儿童ALL预后的关系。方法应用半定量逆转录聚合酶链反应(RT-PCR)的方法，检测儿童ALL初治患者、缓解患者、复发患者及正常对照儿童GYPC基因、TRIP3基因的表达。结果儿童ALL GYPC基因表达：①GYPC基因表达水平在复发患者中最高，不仅高于正常对照组（P<0.01）和完全缓解组（P<0.01），也高于初治组（P<0.01）。②初治患者中，GYPC基因阳性表达者的完全缓解(CR)率与阴性表达者差异无显著性，但未缓解患者治疗前GYPC基因表达水平高于完全缓解患者（P<0.05）。儿童ALL TRIP3基因表达：①TRIP3基因在初治组ALL中的表达水平与复发组ALL中的表达水平相比差异无显著性，在缓解组ALL中表达水平明显高于复发组及初治组（P<0.01）及正常对照组；②在初治的ALL患儿中，TRIP3基因表达阳性者其治疗后的完全缓解率高于表达阴性者（P<0.05）。完全缓解患者治疗前TRIP3基因表达水平高于未缓解患者（P<0.05）。结论GYPC可能是儿童ALL的不利因素，GYPC高表达者疗效差，易复发。TRIP3基因高表达者对化疗药物反应敏感，易于缓解。［中国当代儿科杂志，2009，11（1）：29-32］

Abstract:

OBJECTIVE: To study the relationship of GYPC and TRIP3 gene expression and the prognosis of acute lymphoblastic leukemia (ALL) in children in order to explore the molecular biological mechanisms of recurrence and remission of ALL. METHODS: Thirty-eight newly diagnosed ALL children were enrolled. Of the 38 patients, 31 achieved complete remission (CR) and 12 relapsed. Semi-quantitative RT-PCR was employed to measure blood GYPC and TRIP3 gene expression. Twenty blood samples from normal children were used as controls. RESULTS: Blood GYPC expression in newly diagnosed ALL children was significantly higher than that in the control group (P<0.01) and the CR group (P<0.01). The expression of GYPC gene in the CR group was similar to that in the control group. Other than the control group (P<0.01) and the CR group (P<0.01), the GYPC expression of the relapse group was significantly higher than that in the newly diagnosed group (P<0.01). The CR group showed lower GYPC gene expression than the non-remission group before treatment (P<0.05). Blood expression of TRIP3 gene in the newly diagnosed and the relapse groups was significantly lower than that in the control group (P<0.05). The CR group had increased TRIP3 gene expression compared with the control group (P<0.01) as well as the newly diagnosed and the relapse groups (P<0.01). Of the 38 newly diagnosed ALL children, the patients with positive TRIP3 expression showed higher remission rate than those with negative TRIP3 (P<0.05). The TRIP3 gene expression before treatment in patients who achieved CR was higher than that in non-remission patients (P<0.05). CONCLUSIONS: A high GYPC gene expression is associated with an unfavorable outcome, in contrast, a high TRIP3 gene expression is associated with a favorable outcome in childhood ALL.［Chin J Contemp Pediatr, 2009, 11 (1):29-32］