过敏性紫癜患儿血、尿标本中IL-13受体α2水平及其他细胞因子的检测分析

Roles of serum and urinary interleukins 13Rα2 and other cytokines in pediatric Henoch-Schonlein purpura

摘要:

目的：探讨过敏性紫癜（HSP）患儿血清和尿中IL-13受体α2（IL-13Rα2）与白细胞介素（IL）-4，-6，-8，肿瘤坏死因子α(TNF-α)的关系及其临床意义。方法：采用ELISA法检测了52例HSP患儿(其中29例有肾脏损害)以及45例正常健康儿童的血清和尿IL-13Rα2及IL-4，-6，-8，TNF-α水平，比较上述各组患儿的IL-13Rα2与各细胞因子水平的关系。结果：①HSP患儿的血清中IL-4，IL-6，IL-8，IL-13Rα2及TNF-α水平高于健康对照组(P<0.01或0.05)；HSP患儿尿液中IL-6，TNF-α水平高于正常对照组(P<0.01)。②有肾损害（HSPN）组血清IL-4，-6，-8，IL-13Rα2及TNF-α水平均高于健康对照组；有肾损害（HSPN）组尿液中IL-6，IL-13Rα2及TNF-α水平高于健康对照组。结论：IL-13Rα2，IL-4，-6，-8，TNF-α均可能参与HSP/HSPN的发病过程。［中国当代儿科杂志，2009，11（1）：37-40］

Abstract:

OBJECTIVE: To study the roles of serum and urinary interleukins (IL)-13Rα2, IL-4, IL-6, IL8 and tumor necrosis factor-α(TNF-α) in pediatric Henoch-Schonlein purpura (HSP). METHODS: Serum and urinary levels of IL-13Rα2, IL-4, IL-6, IL-8 and TNF-α were examined using ELISA in 52 children with HSP and 45 healthy children. The results were compared between the two groups. RESULTS: Serum levels of IL-13Rα2, IL-4, IL-6, IL-8 and TNF-α in HSP patients with or without renal lesions were higher than those in the control group (P< 0.01 or 0.05). Urinary levels of IL-6 and TNF-α in HSP patients without renal lesions were higher than those in the control group (P<0.05). Except for urinary levels of IL-6 and TNF-α, urinary IL-13Rα2 levels in HSP patients with renal lesions (HSPN) were higher than those in the control group (P<0.05 ). CONCLUSIONS: Cytokines IL-13Rα2, IL-4, IL-6, IL-8 and TNF-α may play roles in the pathogenesis of pediatric HSP/HSPN.［Chin J Contemp Pediatr, 2009, 11 (1):37-40］