人参皂甙Re对异丙肾上腺素致家兔室性心律失常的保护作用

Protective effect of ginsenoside Re on isoproterenol-induced triggered ventricular arrhythmia in rabbits

摘要:

目的：探讨人参皂甙Re对异丙肾上腺素致家兔实验性室性心律失常的保护作用。方法：异丙肾上腺素诱导产生触发性室性心律失常模型，家兔分为正常对照组、模型组、阳性对照组（异搏定0.4 mg/kg）和实验组（人参皂甙Re 20，10或5 mg/kg），观察转为窦性节律持续时间，实验结束取左心室部分心肌组织行光学显微镜和电子显微镜检查。结果：①异博定组维持窦性节律的时间为177.00±5.66 s，人参皂甙Re 20, 10, 5 mg/kg处理组维持窦性节律的时间分别是177.83±5.31 s、21.00±2.83 s、4.50±1.64 s。②心肌病理切片表明人参皂甙Re对持续室性心律失常的心肌有显著保护作用。结论：人参皂甙Re可用于临床触发性室性心律失常的治疗，可拮抗儿茶酚胺类药物对心肌的毒性作用。［中国当代儿科杂志，2009，11（5）：384-388］

Abstract:

OBJECTIVE: To study the anti-arrhythmic efficacy of ginsenoside Re (GSRe) and its protective effects against myocardial injuries in rabbits with isoproterenol-induced triggered ventricular arrhythmia (TVA). METHODS: TVA model was prepared by intravenous injections of isoproterenol at a constant speed of 5 mg/kg/min. When TVA appeared, rabbits were randomly injected with GSRe (5, 10 or 20 mg/kg), verapamil (0.4 mg/kg) or placebo. The duration of maintaining sinus rhythm was observed. Meanwhile, isoproterenol was continued to be injected at a constant speed of 5 mg/kg/min. After 1 hr of isoproterenol injection, the rabbits were sacrificed. Cardiac muscles in the cuspidate position of the left ventricle were sampled for optical microscopy and electron microscopy. RESULTS: GSRe and verapamil treatment restored sinus rhythm. The duration of sinus rhythm was 177.00±5.66 s within 3 minutes in the verapamil treatment group and was 177.83±5.31, 21.00±2.83 and 4.50±1.64 s, respectively, in the 20, 10 and 5 mg/kg GSRe treatment groups. Histopathologic examination demonstrated that GSRe treatment (20 and 10 mg/kg) alleviated myocardial injuries induced by TVA. CONCLUSIONS: GSRe has anti-arrhythmic efficacies and protective effects against myocardial injuries in rabbits with TVA. It may therefore be a possible therapy for TVA.［Chin J Contemp Pediatr, 2009, 11 (5):384-388］