儿童过敏性紫癜性肾炎血管内皮生长因子基因多态性的研究
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R554+.6

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Gene polymorphism of vascular endothelial growth factor in children with Henoch-Schonlein purpura nephritis
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    摘要:

    目的:探讨血管内皮生长因子(VEGF)-634G/C基因多态性与汉族儿童紫癜性肾炎(HSPN)的关系。方法:应用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)技术对100例过敏性紫癜(HSP)汉族儿童进行VEGF -634G/C基因型分析,其中包括合并紫癜性肾炎50例(HSPN 组),无合并肾炎者 50例(单纯HSP组)。50例年龄、性别匹配的健康汉族儿童作为对照组。并采用酶联免疫吸附试验(ELISA)检测各组血浆VEGF水平。结果:HSPN组VEGF-634CC基因型(32%)和C等位基因频率(56%)均高于对照组(分别为10%和33%)及单纯HSP组(分别为10%和35%,P<0.01)。HSP患儿中,CC基因型者肾炎的发生率比GG基因型者明显增加, 差异有显著性意义(76% vs 31%,P<0.01)。CC基因型者血浆VEGF水平(180.5±40.7 pg/mL)较CG(145.2±48.3 pg/mL)及GG (101.5±26.5 pg/mL)基因型者显著上升, 差异有显著性意义(P<0.05)。结论:VEGF-634G/C基因多态性与儿童HSPN的发生有关。C等位基因可能是儿童HSPN的易感基因。[中国当代儿科杂志,2009,11(6):417-421]

    Abstract:

    OBJECTIVE: To study the relationship of -634G/C gene polymorphism of vascular endothelial growth factor (VEGF) with Henoch-Schonlein purpura nephritis (HSPN) in children. METHODS: One hundred ethnic Han children with HSP, including 50 children with concurrent nephritis (HSPN group) and 50 children without nephritis (HSP without nephritis group), were enrolled. Fifty age-, sex-and ethnics-matched healthy children were used as the control group. VEGF-634G/C genotypes were determined by PCR-RFLP. Plasma VEGF levels were measured using ELISA. RESULTS: CC genotype distribution (32%) and C allele frequency (56%) in the HSPN group were significantly higher than those in the control group (10% and 35% respectively) and the HSP without nephritis group (10% and 33% respectively) (P<0.01). The incidence of nephritis in HSP patients with CC genotype increased significantly when compared with those with GG genotype (76% vs 31%; P<0.01). Plasma VEGF levels in patients with CC genotype (180.5±40.7 pg/mL) were significantly higher than those in patients with CG (145.2±48.3 pg/mL) and GG (101.5±26.5 pg/mL) genotypes (P<0.05). CONCLUSIONS: VEGF-634G/C gene polymorphism may be associated with the development of HSPN. C allele may a susceptible gene of HSPN.[Chin J Contemp Pediatr, 2009, 11 (6):417-421]

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曾华松, 熊小燕, 陈耀勇, 罗小平.儿童过敏性紫癜性肾炎血管内皮生长因子基因多态性的研究[J].中国当代儿科杂志,2009,11(06):417-421

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