癫癎患儿外周血单个核细胞NF-κb活化的变化

NF-kappa B activation in peripheral blood mononuclear cells of children with epilepsy

摘要:

目的：探讨癫癎患儿外周血单个核细胞中核因子κB(nuclear factor-kappa B, NF-κB)活化的变化及其临床意义。方法：采用流式细胞学方法检测84例癫癎患儿治疗前后及32例正常儿的外周血单个核细胞中NF-κB的活化情况。结果：各实验癫癎组患儿外周血单个核细胞中NF-κB的活化均明显高于健康对照组（P<0.05，或<0.01），差异具有显著性意义；全身发作组和分类不明组的癫癎患儿外周血单个核细胞中NF-κB的活化明显高于部分发作组（P<0.01），且全身发作组高于分类不明组（P<0.05）。癫癎患儿部分发作组、全身发作组及分类不明组在治疗后外周血单个核细胞中NF-κB的活化明显降低（P<0.01），差异具有极其显著性意义。结论：癫癎发作患儿外周血单个核细胞NF-κB的激活是增加的，与发作的严重程度呈正相关。［中国当代儿科杂志，2009，11（1）：44-46］

Abstract:

OBJECTIVE: To study the changes and significance of NF-kappa B activation in peripheral blood mononuclear cells (PBMC) of children with epilepsy. METHODS: NF-kappa B activation in PBMC was assayed by the flow cytometry in 32 healthy children and 64 children with epilepsy before and after treatment. The 64 epileptic children were subdivided into three groups: systemic seizure, partial seizure and unknown classification. RESULTS: NF-kappa B activation in PBMC in three epilepsy subgroups were significantly higher than that in healthy controls. The systemic seizure group showed significantly increased NF-kappa B activation in PBMC compared with the partial seizure group (P<0.01) and the unknown classification group (P<0.05). After treatment NF-kappa B activation in PBMC in three epilepsy subgroups was significantly reduced (P<0.01). CONCLUSIONS: NF-kappa B activation in PBMC increased in children with epilepsy, and it was positively correlated with the severity of seizures.［Chin J Contemp Pediatr, 2009, 11 (1):44-46］