苏州地区急性呼吸道感染住院患儿病毒病原学分析

Viral pathogens of acute respiratory infection in hospitalized children from Suzhou

摘要:

目的：了解苏州地区急性呼吸道感染（ARI）住院患儿的病毒病原学情况。方法：采集1 668例7岁以下ARI住院患儿鼻咽分泌物，直接免疫荧光法检测呼吸道合胞病毒（RSV），流感病毒A，B型（IV A，B），副流感病毒1，2，3型（PIV 1，2，3）及腺病毒A(ADA)等7种常见呼吸道病毒，用RT-PCR法检测人类偏肺病毒（hMPV）N基因。结果：① 1 668例患儿中，病毒检测阳性597例（35.8%）。单项病毒感染561例（33.6%），其中7种常见呼吸道病毒检出率为23.1%，以RSV为主（17.6%），好发于冬春季，主要见于1岁以下婴幼儿；② hMPV阳性率为10.6%，3~5月份为检出高峰期。③ 36例混合感染中，22例为RSV合并hMPV感染，主要见于1岁以下婴幼儿。结论：RSV是该地区ARI的主要病毒病原，RSV感染好发于冬春季，多见于1岁以下婴幼儿，hMPV是3~5月份ARI患儿的主要病毒病原，混合感染多见于1岁以下婴幼儿。［中国当代儿科杂志，2009，11（7）：529-531］

Abstract:

OBJECTIVE: To investigate the viral pathogens of acute respiratory infection (ARI) in hospitalized children from Suzhou of China. METHODS: The nasopharyngeal aspirate samples were obtained from 1 668 hospitalized children with ARI. Common respiratory viruses, including respiratory syncytial virus (RSV), influenza virus A and B, parainfluenza viruses 1, 2 and 3 and adenovirus, were detected using direct immunofluorescence. Human metapneumovirus (hMPV) gene fragments were detected by RT-PCR. RESULTS: Viral agents were identified in 597 cases (35.8%). RSV was the most frequent (17.6%). RSV infection is more common in children less than 1 year old. A highest detection rate of RSV was found during winter and spring. hMPV was detected in 10.6% of the cases, with a peak detection rate between March and May. Single viral infection was found in 561 cases (33.6%) and mixed viral infection in 36 cases (including 27 cases at age of less than 1 year). RSV and hMPV co-infection was common (n=22). CONCLUSIONS: RSV is common pathogen of ARI in children from Suzhou. RSV viral activity peaks during winter and spring. The children at age of less than 1 year are susceptible to RSV. hMPV is also an important pathogen of ARI, with a peak detection rate between March and May. Mixed viral infection is common in children less than 1 year old.［Chin J Contemp Pediatr, 2009, 11 (7):529-531］