不同浓度脂多糖诱导人支气管上皮细胞β防御素3的表达

Expression of human β-defensin-3 induced by lipopolysaccharide in human bronchial epithelial cells

摘要:

目的：观察不同浓度脂多糖（LPS）诱导人支气管上皮细胞β防御素3（hBD-3）的表达，探讨hBD-3在呼吸道感染中的作用。方法：用不同浓度的LPS（0.01，0.1，1，10 μg/mL）诱导人支气管上皮细胞后，2 h行RT-PCR法检测hBD-3 mRNA的表达，4 h行Western blot检测hBD-3蛋白的表达。结果：正常人支气管上皮细胞有微量hBD-3表达，LPS诱导细胞后，hBD-3 mRNA及蛋白表达均增加，与对照组相比差异有非常显著性（P＜0.01）；不同浓度LPS诱导细胞后，随着LPS浓度的增加hBD-3 mRNA和蛋白的表达亦随之增加，不同浓度组间比较差异有显著性（P＜0.05）。结论：一定剂量的LPS可诱导人支气管上皮细胞hBD-3表达，且呈浓度依赖性，推测hBD-3可能参与呼吸道对LPS最初的防御反应。［中国当代儿科杂志，2009，11（7）：577-580］

Abstract:

OBJECTIVE: To study the expression of human β-defensin-3 (hBD-3) induced by lipopolysaccharide (LPS) in human bronchial epithelial (HBE) cells, and explore the role of hBD-3 in respiratary infection. METHODS: HBE cells were stimulated with different concentrations of LPS (0.01, 0.1, 1 and 10 μg/mL). hBD-3 mRNA expression was detected by RT-PCR 2 hrs later. hBD-3 protein expression was detected by Western blot 4 hrs later. RESULTS: hBD-3 mRNA and protein was weakly expressed in normal HBE cells. LPS stimulation resulted in a significant increase of hBD-3 mRNA and protein expression (P<0.01). hBD-3 mRNA and protein expression increased with increasing LPS concentrations. There were significant differences in the hBD-3 mRNA and protein expression in cells stimulated by different concentrations of LPS (P<0.05). CONCLUSIONS: LPS can induce hBD-3 expression in a dose-dependent manner. hBD-3 might play a role in initial defensive reaction against bacterial invasion.［Chin J Contemp Pediatr, 2009, 11 (7):577-580］