拓扑替康对HPB-AM细胞MAGE基因表达的影响

Effect of topotecan on MAGE gene expression of HPB-AM cells

摘要:

目的：探讨抗肿瘤新药拓扑替康（TPT）对T细胞淋巴瘤系HPB-AM细胞株黑色素病抗原（MAGE）基因表达的影响。方法：以T细胞淋巴瘤系HPB-AM细胞株为研究对象，采用RT-PCR方法检测TPT作用不同时间、不同浓度时对HPB-AM细胞株MAGE基因表达的影响。结果：以0.05 μmol/L， 0.10 μmol/L， 0.15 μmol/L和0.20 μmol/L终浓度的TPT作用于HPB-AM细胞，作用12 h后检测MAGE mRNA，随着TPT浓度的增加，MAGE-3 mRNA和MAGE-4 mRNA的表达水平均明显下调，实验组与空白对照组相对表达量比较，在浓度为0.10，0.15和0.20 μmol/L的TPT作用后差异具有显著性意义(P<0.05)。用浓度0.10 μmol/L的TPT作用于HPB-AM细胞系，作用4，8，12，16 h后检测MAGE mRNA，随着TPT作用时间增长，MAGE-3 mRNA和MAGE-4 mRNA的表达水平明显下调。作用12 h和16 h后，实验组与空白对照组相对表达量比较差异具有显著性意义(P<0.05)。结论：拓扑替康能抑制HPB-AM细胞中MAGE mRNA的表达，且呈剂量和时间依赖性。［中国当代儿科杂志，2009，11（8）：679-682］

Abstract:

OBJECTIVE: To investigate whether topotecan, a novel anti-tumor angent, down-regulates gene expression of melanoma antigen-encoding (MAGE) in HPB-AM cells. METHODS: MAGE mRNA expression of HPB-AM cells was detected by RT-PCR 4, 8, 12 and 16 hrs after different concentrations (0.05, 0.10, 0.15 and 0.20 μmol/L) of topotecan treatment. RESULTS: MAGE mRNA expression of HPB-AM cells decreased with increasing concentrations of topotecan 12 hrs after treatment. The MAGE mRNA expression of HPB-AM cells treated by 0.10, 0.15 and 0.20 μmol/L of topotecan was significantly lower than that in the blank control group (P<0.05). MAGE mRNA expression of HPB-AM cells was significantly reduced in a time-dependent manner after 0.10 μmol/L of topotecan treatment. The MAGE mRNA expression of HPB-AM cells treated by 0.10 μmol/L of topotecan was significantly lower than that in the blank control group 12 and 16 hrs after treatment (P<0.05). CONCLUSIONS: Topotecan is capable of inhibiting the expression of MAGE mRNA of HPB-AM cells in a time- and dose-dependent manner.［Chin J Contemp Pediatr, 2009, 11 (8):679-682］