川芎嗪对柯萨奇B3病毒感染乳鼠心肌细胞的保护作用及信号转导机制研究

Protective effects of tetramethylpyrazine on rat myocardial cells infected by Coxsackie virus B3 and its signal transduction mechanism

摘要:

目的：临床显示川芎嗪对病毒性心肌炎有治疗作用，但机制不明，该研究拟探讨川芎嗪对病毒感染的心肌细胞的作用及信号转导机制。方法：利用培养的Sprague-Dawley乳鼠心肌细胞，分为空白对照组（心肌细胞正常培养）、阴性对照组（心肌细胞加入川芎嗪至终浓度100 μmol/L后培养）、病毒感染组［心肌细胞感染柯萨奇病毒B3(CVB3)］、川芎嗪干预组(心肌细胞感染病毒后加入终浓度100 μmol/L川芎嗪干预)。观察心肌细胞搏动频率及培养液中乳酸脱氢酶(LDH)活性。采用Western blot测定NF-κB蛋白表达变化，MTT法检测心肌细胞活性。结果：病毒感染组与川芎嗪干预组比较，心肌细胞搏动频率明显降低(32.0±3.6次/min vs 84.3±3.5次/min, P<0.01)，细胞病变加重，LDH活性显著升高(P<0.05)。川芎嗪干预组细胞NF-κB蛋白表达明显低于病毒感染组(P<0.01)，存活率明显高于病毒感染组［(86.7±2.7）％ vs （35.3±3.4）%, P<0.01］。结论：在病毒性心肌炎时川芎嗪可以抑制NF-κB的表达，起到保护心肌细胞的作用。［中国当代儿科杂志，2009，11（8）：687-690］

Abstract:

OBJECTIVE: To study the protective effects of tetramethylpyrazine (TMPZ) on rat myocardial cells infected by Coxsackie virus B3 (CVB3) and its signal transduction mechanism. METHODS: The cultured myocardial cells of neonatal Sprague-Dawley rats were randomly treated with CVB3, CVB3+TMPZ (100 μmol/L), TMPZ (100 μmol/L) (negative control) or DMEM (blank control). After treatment, the beating rate of myocardial cells and the LDH activity in the culture fluid were measured. Cell viability was ascertained with MTT assay. Western blot was used to study the expression of nuclear factor kappa-B (NF-κB) protein in myocardial cells. RESULTS: The beating rate of myocardial cells in the untreated CVB3 infection group was significantly lower than that in the TMPZ-treated CVB3 infection group (32.0±3.6 bpm vs 84.3±3.5 bpm, P<0.01). The LDH activity and NF-κB expression in the TMPZ-treated CVB3 infection group was significantly reduced when compared with untreated CVB3 infection group (P<0.05, P<0.01, respectively). Cell viability 7 days after CVB3 infection in the TMPZ-treated group was higher than that in the untreated CVB3 infection group ［(86.7±2.7)％ vs (35.3±3.4)%; P<0.01］. CONCLUSIONS: TMPZ can provide protective effects on rat myocardial cells infected by CVB3, possibly by an inhibition of the activity of NF-κB in myocardial cells.［Chin J Contemp Pediatr, 2009, 11 (8):687-690］