TNF-α-308G/A和IL-6-174G/C基因多态性与呼吸道合胞病毒毛细支气管炎的相关性

Association of tumor necrosis factor-α-308G/A and interleukin-6-174G/C gene polymorphisms with the susceptibility of respiratory syncytial virus bronchiolitis

摘要:

目的：探讨肿瘤坏死因子(TNF)-α-308G/A、白细胞介素(IL)-6-174G/C基因多态性与呼吸道合胞病毒((RSV) 毛细支气管炎易感性的关系。方法：应用聚合酶链反应限制性酶切片段长度多态性技术（PCR-RFLP）检测150例RSV毛细支气管炎患儿(病例组)和120例正常对照儿童(对照组)TNF-α-308G/A和IL-6-174G/C的基因多态性。结果：①病例组TNF-α-308位点GG、GA、AA基因型频率分别为68.0%、28.0%、4.0%，G、A等位基因型频率分别为82.0%，18.0%；对照组TNF-α-308位点GG，GA，AA基因型频率分别为80.8%，19.2%，0,G、A等位基因型频率分别为90.4%，9.6%；两组基因型及等位基因频率差异有显著性意义，病例组A等位基因的频率明显高于对照组(χ2=5.665，7.726，均P＜0.05)；与G等位基因携带者相比，A等位基因携带者患RSV毛细支气管炎的风险增加了2.071倍(OR=2.071, P＜0.05)；②病例组和对照组IL-6-174G/C位点均只见GG基因型。结论：TNF-α-308A等位基因与RSV毛细支气管炎的易感性相关，其可能是影响RSV毛细支气管炎发病的一个重要候选基因；温州地区汉族儿童未发现IL-6-174G/C基因多态性。［中国当代儿科杂志，2009，11（10）：821-824］

Abstract:

OBJECTIVE: To study the association of tumor necrosis factor (TNF)-α-308G/A and interleukin (IL)-6-174G/C gene polymorphisms with the susceptibility of respiratory syncytial virus (RSV) bronchiolitis. METHODS: Polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) was used to identify the polymorphisms at position 308 of the TNF-α promoter and -174 of the IL-6 promoter in 150 children with RSV bronchiolitis and 120 healthy children (control group). RESULTS: The frequency of GG, GA and AA genotypes of TNF-α-308 was 68.0%, 28.0% and 4.0% respectively in the RSV group, while that was 80.8%, 19.2% and 0 respectively in the control group. There were significant differences in the genotype frequencies between the two groups (χ2=5.665, P<0.05). The frequency of G and A alleles in the RSV group was 82.0% and 18.0% respectively, while that was 90.4% and 9.6% respectively in the control group. The frequency of A allele in the RSV group was significantly higher than that in the control group (χ2=7.726, P<0.05). Compared to the children carrying G allele, the children with A allele demonstrated a 2.071-fold increased risk of RSV bronchiolitis (OR=2.071, P<0.05). Only GG genotype was detected at position -174 of IL-6 in both groups. CONCLUSIONS: TNF-α-308A is associated with the susceptibility of RSV bronchiolitis and it might be an important candidate gene for the development of RSV bronchiolits. IL-6-174G/C gene polymorphism is not found in children in Wenzhou area.［Chin J Contemp Pediatr, 2009, 11 (10):821-824］