哮喘患儿CD4+CD25+调节性T细胞及IL-10和TGF-β1的变化

Changes of CD4+CD25+ regulatory T cells, IL-10 and TGF-β1 levels in peripheral blood in children with asthma

摘要:

目的：通过测定和分析哮喘急性发作期与缓解期儿童外周血中CD4⁺CD25⁺调节性T淋巴细胞及其相关细胞因子白细胞介素-10（IL-10）和转化生长因子-β（TGF-β1）在哮喘发病中的变化情况，探讨哮喘发作的新的免疫机制。方法：分别采集74例哮喘发作期和28例哮喘缓解期患儿及20例健康儿童外周静脉血，通过流式细胞仪分析及酶联免疫吸附试验（ELISA）测定CD4⁺CD25⁺调节性T细胞占CD4⁺T细胞的百分比和血浆中IL-10和TGF-β1水平。结果：①哮喘发作期患儿外周血中CD4⁺CD25⁺调节性T细胞百分率及IL-10水平较缓解期及对照组明显降低（P<0.01），而哮喘缓解期明显升高接近正常水平； ②哮喘急性发作期患儿TGF-β1水平较缓解期及对照组明显升高（P<0.05）。哮喘缓解期患儿TGF-β1水平明显降低，仍高于对照组水平； ③哮喘发作期、缓解期及对照组各组之间CD4⁺CD25⁺T细胞百分率与IL-10、TGF-β1无相关性（P>0.05）。结论：哮喘发作期患儿CD4⁺CD25⁺调节性T细胞和IL-10数量和分泌水平降低、TGF-β1水平增高，导致了全身和局部对炎症反应的抑制作用减弱，可能是导致哮喘发作的原因之一。［中国当代儿科杂志，2009，11（10）：829-832］

Abstract:

OBJECTIVE: This study determined the changes of CD4⁺CD25⁺ regulatory T cells, IL-10 and TGF-β1 levels in peripheral blood in children with asthma in order to explore immunologic mechanism of asthma attacks. METHODS: Seventy-four children with asthma attacks, twenty-eight children with stable asthma and twenty healthy children were enrolled. The percentage of CD4⁺CD25⁺ regulatory T cells in CD4⁺ T cells in peripheral blood was detected by flow cytometry. Serum levels of IL-10 and TGF-β1 were measured using enzyme linked immunosorbent assay (ELISA). RESULTS: The percentage of CD4⁺CD25⁺ regulatory T cells and IL-10 levels in peripheral blood in the asthma attacks group were significantly lower than those in the stable asthma and the control groups (P<0.01) .The stable asthma group showed a similar percentage of CD4⁺CD25⁺ regulatory T cells and IL-10 levels to the control group. Compared with the stable asthma and the control groups, serum TGF-β1 level in the asthma attacks group increased significantly (P<0.05). In the asthma attacks, the stable asthma, and the control groups, the percentage of CD4⁺CD25⁺ regulatory T cells in peripheral blood was not correlated to serum levels of IL-10 and TGF-β1. CONCLUSIONS: The percentage of CD4⁺CD25⁺ regulatory T cells in peripheral blood and serum IL-10 levels decrease and serum TGF-β1 levels increases in children with asthma attacks, which results in a decreased immunosuppressive effect. This might contribute to one of the immunologic mechanisms of asthma attacks.［Chin J Contemp Pediatr, 2009, 11 (10):829-832］