急性淋巴细胞白血病患儿WAVE1和p22phox的表达及WAVE1与氧化应激的关系

Expression of WAVE1 and p22phox in children with acute lymphocytic leukemia and the relationship of WAVE1 with oxidative stress

摘要:

目的：研究儿童急性淋巴细胞白血病（ALL）患者外周血单个核细胞(PBMCs)中WASP家族富含脯氨酸同源蛋白1（WAVE1）及NADPH氧化酶亚基p22phox的表达并探讨WAVE1表达与白血病病程及氧化应激的关系。方法：实时定量PCR法测定41例ALL儿童及10例正常对照儿童PBMCs中WAVE1和p22phox的表达水平。黄嘌呤氧化酶法（羟胺法）和二硫代二硝基苯甲酸法（DTNB法）分别测定外周血血浆总超氧化物歧化酶（SOD）活性和谷胱甘肽过氧化物酶（GSH-Px）活性。结果：①儿童ALL活动期组（初治+复发）PBMCs中WAVE1，p22phox的表达水平与正常对照组和完全缓解组相比均显著增高(P< 0.01)； ALL完全缓解组中，WAVE1，p22phox表达高于正常对照组(P<0.05)；②正常对照组、活动期组和缓解组血浆SOD活力分别为166.35±27.93, 22.62±7.39, 107.11±28.57 U/mL; GSH-Px活力分别为490.94±39.38，91.73±28.88，267.56±82.64 μmol/L。ALL活动期组外周血血浆总SOD,GSH-Px活性较完全缓解和正常儿童明显降低（P<0.01）；③ 随着WAVE1和p22phox的高表达，血浆中SOD, GSH-Px活性呈现下降趋势。WAVE1的表达水平与p22phox表达水平呈正相关（r=0.34，P< 0.05），与血浆SOD，GSH-Px活性呈负相关（r分别为-0.336和-0.408，P<0.05）。结论：与正常对照组相比，WAVE1和p22phox在儿童ALL PBMCs中表达增高，而且其变化与ALL病程相关。ALL患儿氧化应激可能参与了WAVE1表达的调控。［中国当代儿科杂志，2009，11（2）：88-92］

Abstract:

OBJECTIVE: To study the expression of WAVE1 and p22phox in peripheral blood mononuclear cells (PBMCs) in children with acute lymphocytic leukemia (ALL) and the relationship of WAVE1 with oxidative stress. METHODS: Real-time PCR was used for detecting WAVE1 and p22phox expression in PBMCs in 41 children with ALL and 10 normal controls. Plasma activity of superoxide dismutase (SOD) was measured by the xanthine oxidase method. Plasma activity of GSH-Px was measured by the DTNB reaction test. RESULTS: The expression of WAVE1 and p22phox was significantly higher in the active ALL groups (newly diagnosed and relapse ALL) than that in the normal control and the complete remission (CR) ALL groups (P<0.01). The CR ALL group showed increased WAVE1 and p22phox expression than those in the normal control group (P<0.05). Plasma activities of SOD (22.62±7.39 U/mL) and GSH-Px (91.73±28.88 μmol/L) in the active ALL group were significantly lower than those in the normal control (166.35±27.93 U/mL and 490.94±39.38 μmol/L, respectively) and the CR ALL groups (107.11±28.57 U/mL and 267.56±82.64 μmol/L, respectively) (P<0.01). WAVE1 expression was positively correlated with p22phox expression (r=0.34, P<0.05) but negatively correlated with plasma activities of SOD and GSH-Px ( r=-0.336 and-0.408, respectively; P<0.05). CONCLUSIONS: WAVE1 and p22phox expression in PBMCs increased and was associated with the disease course in children with ALL. Oxidative stress may be involved in the regulation of WAVE1 expression in ALL children.［Chin J Contemp Pediatr, 2009, 11 (2):88-92］