电刺激致痫大鼠惊厥阈下脑放电动物模型的建立

摘要:

目的 建立惊厥阈下脑放电动物模型，研究惊厥阈下脑放电（ＳＣＤ） 对大鼠空间变换学习记忆的影响。方法 选用雄性Ｗｉｓｔａｒ 大鼠１８ 只，采用慢性电极埋植法，在连续ＥＣｏＧ 记录下给予大鼠前额叶皮层电刺激，同时观察大鼠行为及脑电变化。结果 当３０ ～５０ μＡ 电刺激时可在额叶皮层引起４ ．５～５ Ｈｚ 单发或连续的高波幅棘慢波，海马则为与皮层同步的高波幅θ节律，无临床惊厥表现。结论 ３０ ～５０ μＡ 电刺激可引起皮层和海马的惊厥阈下放电。

Abstract:

Objective To set up an animal model of subthreshold convulsant discharges (SCD) and to study the effects of SCD on learning and memory ability of space changes in rats.Methods Chronic electrodes were placed in the anterior frontal cortex of 18 male Wistar rats. Under continuous ECoG recording, electrial stimulation was given to the anterior frontal cortex of the rats. Changes of behaviors and ECoG of the rats were observed simultaneously. Results Epileptiform EEG discharges in the cortex and hippocampus were induced without seizure during the subconvulant electric stimulation (SES) to the anterior frontal cortex. Conclusions The anterior frontal cortex SES can cause SCD in the cortex and hippocampus.