复方丹参注射液对小鼠心肌保护作用的研究

摘要:

目的 探讨复方丹参注射液对小鼠心肌的保护作用。方法 ２５ 只小鼠，随机分成缺氧组，丹参治疗组和对照组，实验２ 周后，取新鲜心肌标本，测琥珀酸脱氢酶（ＳＤＨ），三磷酸腺苷（ＡＴＰ） 酶，乳酸脱氢酶（ＬＤＨ），应用光镜和电镜观察心肌病理性变化。结果 缺氧组ＳＤＨ 和ＡＴＰ酶减少，ＬＤＨ 升高，心肌间质水肿，心肌细胞线粒体肿胀，肌丝排列紊乱，丹参治疗组，心肌酶和心肌超微结构与对照组相比均无显著性差异。结论 复方丹参对小鼠心肌缺氧损伤有保护作用。

Abstract:

Objective To determine the protective effects of Danshen complex injection against hypoxia-mediated injury in myocaridium.Methods Twenty-five mice were divided into normal control (CON) , hypoxia (HYP) , and Danshen (DAN) treatment groups. Myocardium samples were taken at two weeks after the start of experimental challenge. Myocardium succinic dehyrogenase (SDH), adenosine triphosphatase (ATP) and lactic dehydrogenase (LDH) were measured. Pathologic changes of myocardium were observed under the light and electric microscopes.Results In HYP, SDH and ATP drecrease and LDH increase were observed ; in addition, the mesenchyma of the myocardium was edematous and mitochondrions of myocardial cells were enlarged ; the arragement of filaments was irregular. However, there was no significant difference in myocardium enzyme levels and morphological structure between DAN and CON groups.Conclusions Enzymohistochemical and morphological findings in this study suggest that hypoxia causes myocardium impairment in mice and that Danshen can protect myocardium against hypoxia-induced injury.