IL-13鳞状细胞癌抗原及IgE在儿童咳嗽变异性哮喘中的检测价值

Levels of serum IL-13, squamous cell carcinoma antigen and IgE in children with cough variant asthma

摘要:

目的 咳嗽变异性哮喘是一种与白细胞介素 13(IL 13)相关的以气道炎症为特征的疾病。研究表明 ,IL 13所诱导的基因中鳞状细胞癌抗原 (SccAg)表达最多 ,因此推测SccAg在咳嗽变异性哮喘中的发病中也起重要作用。本文旨在评价IL 13、SccAg及免疫球蛋白E(IgE)在咳嗽变异性哮喘患儿中的检测价值。 方法 用酶联免疫吸附试验 (ELISA)法检测 5 1例咳嗽变异性哮喘患儿、2 6例哮喘患儿、33例正常儿童血清IL 13、SccAg及IgE水平 ,并对结果进行统计学处理。 结果 ①咳嗽变异性哮喘患儿发作期血清IL 13(2 38.88± 4 0 .0 7ng/L)、SccAg (2 .81± 0 .38ng/ml)水平显著高于缓解期 (85 .15± 17.98ng/L,2 .2 9± 0 .31ng/ml)及正常对照组 (77.2 7±18.16ng/L,2 .2 9± 0 .34ng/ml) (均P <0 .0 1) ,但缓解期及正常对照组间差异无显著性 ;②咳嗽变异性哮喘发作期患儿血清IgE (6 2 2 .4 8± 2 95 .0 1KU/L)水平显著高于缓解期 (373.81± 15 7.92KU/L) ,两组均显著高于正常对照组 (10 2 .99± 38.81KU/L) (均P <0 .0 1) ;③咳嗽变异性哮喘患儿发作期血清IL 13、SccAg及IgE水平与哮喘患儿发作期 (2 6 3.12± 4 9.99ng/L ,3.0 1± 0 .37ng/ml,717.0 4± 314 .0 1KU/L)间差异无显著性。结论 联合检测血清IL 13、SccAg及IgE水

Abstract:

Objective Cough variant asthma (CVA) is correlated with interleukin-13 (IL-13) and characterized by inflammation of the air way. Among the products induced by IL-13, squamous cell carcinoma antigen (SccAg) is of the highest amount, which suggests that SccAg may play a role in the pathogenesis of CVA. This study aims to evaluate the values of IL-13, SccAg and immunoglobulin E (IgE) in CVA. Methods Serum IL-13, SccAg and IgE levels were measured using enzyme-linked immunosorbent assay (ELISA) in 51 children with CVA (CVA group), 26 with asthma (Asthma group) and 33 normal children (Normal control group). Results ① In the CVA group, serum levels of IL-13 ( 238.88± 40.07 ng/L) and SccAg ( 2.81± 0.38 ng/ml) on the acute phase were significantly higher than those on the remission phase ( 85.15± 17.98 ng/L and 2.29± 0.31 ng/ml, respectively; P< 0.01). No differences were found between the remission phase patients from the CVA group and the Normal control group. ② There were significant differences in the serum IgE level between the patients from the acute and remission phases in the CVA group ( 622.48± 295.01 KU/L vs 373.81± 157.92 KU/L; P< 0.01), and the serum levels of both the acute and remission phases were significantly higher than that of the Normal control group ( 102.99± 38.81 KU/L; both P< 0.01). ③ The serum IL-13, SccAg and IgE levels in the CVA group on the acute phase were not statistically different from those of the Asthma group. Conclusions The detection of serum IL-13, SccAg and IgE levels may be valuable in the diagnosis and treatment of CVA. IL-13 and SccAg may play a role in the genesis of CVA. The pathogenesis of CVA might be identical to asthma, and CVA seems to be a pioneer manifestation of typical asthma.