癫痫和热性惊厥病儿一氧化氮一氧化氮合酶的研究

Levels of nitric oxide and nitric oxide synthase in serum and cerebrospinal fluid of children with epilepsy or febrile convulsions

摘要:

目的 探讨癫痫 (EP)和热性惊厥 (FC)病儿血清和脑脊液 (CSF)一氧化氮 (NO)和一氧化氮合酶(NOS)的含量及其与EP和FC的发病机制的关系。方法 分别采用硝酸还原酶法、酶测定法测定EP和FC病儿惊厥发作后 2 4h内血清和CSFNO和NOS的含量。结果 EP组和FC组CSFNO水平均明显低于对照组 ,差异有显著性 (P <0 .0 5 ) ;而EP组和FC组血清NO水平均明显高于对照组 ,差异有显著性 (P <0 .0 5 )。FC组CSFNOS活性也明显低于对照组 (P <0 .0 5 )。结论 EP和FC病儿CSFNO水平明显低于对照组 ,提示NO可能与阻止惊厥发作有关 ,并可能有抗惊厥作用。FC病儿CSFNOS活性明显低于对照组 ,与FC病儿CSFNO水平下降一致 ,进一步支持NO在FC中可能有抗惊厥作用 ,这一结果可为EP的临床药物治疗和研制开发提供新的思路。

Abstract:

Objective To examine nitric oxide (NO) and nitric oxide synthase (NOS) concentrations in serum and cerebrospinal fluid (CSF) of children with epilepsy (EP) or febrile convulsions (FC) and to investigate the role of NO in the pathogenesis of EP and FC. Methods The serum and CSF concentrations of NO and NOS in children with EP (EP group, n=31) or FC (FC group, n=39) were examined by nitrate reductase and enzyme determination assays respectively within 24 hours after seizures. The results were compared with 38 control children with upper respiratory infection. Results Both EP and FC groups demonstrated a lower CSF NO levels compared with the Control group (P< 0.05). In contrast, serum NO levels in both groups were significantly higher than those in the Control group (P< 0.05). CSF levels of NOS in the FC group decreased significantly when compared with the Control group (P< 0.05). Conclusions CSF NO levels decreased after seizures in children with either EP or FC. CSF levels of NOS also decreased in the FC children. The results indicate a possible role of NO in anti-convulsion.