支气管哮喘模型大鼠IL-4 IL-10 IFN-γ的检测

Levels of IL24 , IL210 and IFN2γ in rats with asthma

摘要:

目的 哮喘的发病机理尚不完全清楚 ,近年来 ,细胞因子在哮喘中的作用引起人们的关注。本文旨在探讨支气管哮喘的免疫学发病机理 ,研究细胞因子IL 4、IL 10、IFN γ与哮喘的关系。方法 建立大鼠支气管哮喘模型。将Wistar大鼠随机分为两组 :正常对照组 (n =11)及哮喘组 (n =11) ,以卵白蛋白 (OVA)腹腔注射致敏大鼠 ,两周后雾化吸入OVA发敏 ;发敏后杀鼠 ,取脾 ,制备单个核细胞悬液 ,体外培养 ,并以OVA刺激 ,取不同时间培养上清 ,应用ELISA双抗体夹心法测定其中IL 4、IL 10和IFN γ的水平。结果 支气管哮喘组动物单个核细胞培养上清中IL 4的水平明显高于正常对照组 (6 4 .5 6± 5 .83vs 2 4 .6 6± 3.6 8pg/ml,P <0 .0 5 ) ,IL 10水平明显低于正常对照组 (34.13± 0 .85vs 85 .12± 6 .13,P <0 .0 5 ) ,IFN γ水平明显低于正常对照组 (3.87± 0 .4 8vs14 .5 1± 1.32 pg/ml,P <0 .0 5 )。结论 细胞因子IL 4、IL 10和IFN γ可能参与了哮喘发病的病理生理过程。

Abstract:

Objective The pathogenic mechanism of asthma has not yet been explained. This paper aims to study the relationships between IL-4, IL-10 and IFN-γ and asthma so as to explore the immunologic pathogenesis of asthma. Methods The Wistar rats were randomly assigned into 2 groups: a Normal group (n=11) and an Asthma group (n=11). Asthma was induced by intraperitoneal injection of OVA and fog inhalation of OVA. Five days after asthma induction, the rats were sacrificed and their spleens were removed. Then the mononuclear cell suspension was prepared, cultured, and stimulated with OVA. The levels of IL-4, IL-10 and IFN-γ in the supernatant were measured by enzyme linked immunosorbent assay (ELISA). Results The IL-4 level in the mononuclear cells of the Asthma group ( 64.56± 5.83 pg/ml) was significantly higher than that of the Normal group ( 24.66± 3.68 pg/ml) (P< 0.05), while the levels of IL-10 ( 34.13± 0.85 pg/ml) and IFN-γ ( 3.87± 0.48 pg/ml) were significantly lower than those of the Normal group [( 85.12± 6.13) and ( 14.51± 1.32) pg/ml, respectively] (P< 0.05). Conclusion IL-4, IL-10 and IFN-γ might be involved in the pathogenesis of asthma.