苦参碱对急性淋巴细胞性白血病细胞Bcl-2表达的影响

The effect of matrine on Bcl22 expression in acute lymphoblastic leukemia cells

摘要:

目的 苦参碱是苦参的主要成分 ,具有抗K5 6 2细胞、HL 6 0细胞增殖的作用。本文主要探讨苦参碱抗儿童急性淋巴细胞白血病 (ALL)细胞增殖作用及可能机制。方法 MTT法检测苦参碱对ALL细胞的生长抑制作用 ,流式细胞仪 (FCM)检测苦参碱对ALL细胞Bcl 2表达的影响。结果 0 .1mg/ml～ 1.0mg/ml浓度的苦参碱对ALL细胞的增殖有抑制作用。培养 4 8h后 ,与未处理组相比 ,其OD值 (MTT法 )由 0 .84± 0 .2 7分别降低为 0 .1mg/ml时的 0 .73± 0 .16 ,0 .2mg/ml时的 0 .5 8± 0 .15 ,0 .5mg/ml时的 0 .32± 0 .16 ,1.0mg/ml时的 0 .2 4± 0 .14 ,差异有显著性。苦参碱可下调ALL细胞Bcl 2的表达 ,培养 4 8h后 ,Bcl 2阳性率由 (2 5 .0± 5 .8) %降低至0 .1mg/ml时的 (2 1.3± 6 .1) % ,0 .2mg/ml时的 (18.2± 3.7) % ,0 .5mg/ml时的 (14 .5± 4 .0 ) % ,1.0mg/ml 时的(10 .0± 3.2 ) %。与对照组相比差异有显著性。结论 苦参碱具有抗ALL细胞的增殖作用 ,其可能机制可能与其下调ALL细胞Bcl 2的表达有关。

Abstract:

Objective Matraine, a main ingredient of sophora, can inhibit the proliferation of many tumor cells such as K562 and HL-60. This study was designed to investigate the effect of matrine on acute lymphoblastic leukemia (ALL) cells and its possible mechanisms. Methods The samples were obtained from 24 children with initial ALL. The ALL cells were co-cultured with matrine. The inhibition effect of the proliferation of ALL cells was detected by the MTT assay. A flow cytometer (FCM) was used to detect the expression of Bcl-2 in ALL cells. Results Compared with the controls without matrine treatment ( 0.84± 0.27), the OD values (reflecting the survival cell amount) were reduced significantly after 48 hrs of different concentrations of matrine incubation ( 0.73± 0.16 at 0.1 mg/ml, 0.58± 0.15 at 0.2 mg/ml, 0.32± 0.16 at 0.5 mg/ml, and 0.24± 0.14 at 1.0 mg/ml). The positive rate of Bcl-2 protein was down-regulated significantly from ( 25.0± 5.8)% in the untreated controls to ( 21.3± 6.1)% in the cells treated with 0.1 mg/ml matrine, and ( 18.2± 3.7)% for 0.2 mg/ml, ( 14.5± 4.0)% for 0.5 mg/ml and ( 10.0± 3.2)% for 1.0 mg/ml at 48 hrs of incubation. Conclusions Matraine can inhibit the proliferation of ALL cells, and the possible mechanism may be associated with the down-regulated Bcl-2 expression in ALL cells.